Role of hemeoxygenase‐2 in pregnancy‐induced hypertension
Role of hemeoxygenase‐2 in pregnancy‐induced hypertension
复制标题
血红素加氧酶-2在妊娠高血压综合征中的作用
DOI：
10.1016/s0020-7292(03)00196-6
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发表时间：
2004
影响因子：
3.8
通讯作者：
H. Chen
中科院分区：
文献类型：
--
作者：
D. Shrestha Dangol;H. Chen
应用免疫组织化学和高分辨率病理图像分析系统(HPIAS-1000)观察了56例足月妊娠、42例诊断为妊高征(PIH)患者和14例正常妊娠胎盘组织中HO-2的表达水平。统计分析采用方差分析和组后检验(Tukey)。PF0。05被认为是重要的。HO-2是血红素加氧酶同工酶之一，在NADPH和细胞色素P-450存在下催化血红素氧化生成一氧化碳(CO)和胆红素w1x。它们被认为促进血管扩张和保护内皮细胞和其他细胞免受氧化损伤，分别为w2，3x。在本研究中，我们发现HO-2在正常妊娠中的表达在合体滋养层细胞(STB)和绒毛内皮细胞(图1)中呈暗褐色。然而，在中度和重度妊高征患者的这些细胞中发现了中断的淡棕色(图2)。HO-2在中、重度妊高征胎盘STB和内皮细胞中的表达水平低于正常妊娠
We conducted this study to observe the level of hemeoxygenase-2 (HO-2) expression in placentas of 56 term pregnant women, 42 with a diagnosis of pregnancy-induced hypertension (PIH) and 14 normal pregnancies by using immunohistochemistry and a high resolution pathological image analysis system (HPIAS-1000). ANOVA and the post-hoc test (Tukey) were used for statistical analysis. PF0. 05 was considered significant. HO-2 is one of the isoenzyme of hemeoxygenase which catalyses the oxidation of heme in the presence of NADPH and cytochrome P-450 to produce carbon monoxide (CO) and bilirubin w1x. They are considered to promote vasodilation and protection of endothelium and other cells from oxidative injury, respectively w2, 3x. In this study we found that the expression of HO-2 in normal pregnancy revealed a dark brownish-yellow color in the syncytiotrophoblast (STB) and villous endothelial cells (Fig. 1). However, interrupted light brownish color was noted in these cells of moderate and severe PIH women (Fig. 2). The level of HO-2 expression in STB and endothelial cells of moderate and severe PIH placentas were reduced compared with normal pregnancy