Expression of insulin-like growth factor-I during chicken development.
Expression of insulin-like growth factor-I during chicken development.
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DOI：
10.1210/endo-128-3-1323
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发表时间：
1991-03
期刊：
影响因子：
4.8
通讯作者：
K. Kikuchi;F. Buonomo;Y. Kajimoto;P. Rotwein
中科院分区：
文献类型：
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作者：
K. Kikuchi;F. Buonomo;Y. Kajimoto;P. Rotwein
我们研究了雏鸡个体发育过程中胰岛素样生长因子-I (IGF-I) 的表达。在第 6 天，首次在整个胚胎中检测到 IGF-I mRNA，而在第 9 天（检测的最早时间点）可以测量血清 IGF-I。血清 IGF-I 值从第 10 天的约 3 ng/ml 上升到第 15-17 天期间的孵化前峰值 30-35 ng/ml，上升了 10 倍，然后在孵化时下降至约 10 ng/ml。在孵化第 17 天和第 20 天，在眼睛、骨骼肌和大脑中检测到 IGF-I mRNA，但直到孵化后才在肝脏或心脏中检测到。在孵化后期间，血清IGF-I从第一周的10 ng/ml上升到3-6周的35-40 ng/ml，肝脏IGF-I mRNA从1-7周增加近5倍。第 1-3 周期间观察到的增加与孵化后血清 GH 从 21 ng/ml 上升至 37 ng/ml 相关，尽管 GH 水平在随后的 4 周内下降，而血清 IGF-I 值没有明显变化。相比之下，在胚胎第 12 天之前，循环中无法检测到 GH，而在第 11 天时 IGF-I 为 19 ng/ml。这些观察结果表明，正如在哺乳动物中所发现的那样，小鸡中 IGF-I 基因表达存在 GH 依赖性和 GH 非依赖性调节，并支持 IGF-I 在鸡胚胎发育中发挥作用的观点。
We have investigated the expression of insulin-like growth factor-I (IGF-I) during ontogeny in the chick. IGF-I mRNA was first detectable in whole embryos on day 6, while serum IGF-I could be measured on day 9, the earliest time point examined. Serum IGF-I values rose 10-fold from about 3 ng/ml on day 10 to a prehatch peak of 30-35 ng/ml during days 15-17, and then declined to about 10 ng/ml at the time of hatching. On days 17 and 20 of incubation, IGF-I mRNA was detected in eye, skeletal muscle, and brain, but could not be found in liver or heart until after hatching. During the posthatch period, serum IGF-I rose from 10 ng/ml in the first week to 35-40 ng/ml during weeks 3-6, and liver IGF-I mRNA increased nearly 5-fold from weeks 1-7. The increases observed during weeks 1-3 correlated with a posthatch rise in serum GH from 21 to 37 ng/ml, although GH levels declined over the subsequent 4 weeks without an appreciable change in serum IGF-I values. By contrast, before embryonic day 12 no GH could be detected in the circulation, while IGF-I was 19 ng/ml on day 11. These observations suggest that there is both GH-dependent and GH-independent regulation of IGF-I gene expression in the chick, as has been found in mammals, and support the idea that IGF-I plays a role in chicken embryonic development.