Survival and bioconversion of n-3 fatty acids during early development of dolphin (Coryphaena hippurus) larvae fed oil-enriched rotifers.
Survival and bioconversion of n-3 fatty acids during early development of dolphin (Coryphaena hippurus) larvae fed oil-enriched rotifers.
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喂食富油轮虫的海豚 (Coryphaena hippurus) 幼虫早期发育过程中 n-3 脂肪酸的生存和生物转化。
DOI：
10.1016/0044-8486(90)90132-7
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发表时间：
1990
期刊：
影响因子：
--
通讯作者：
S. Divakaran
中科院分区：
文献类型：
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作者：
A. Ostrowski;S. Divakaran
使用富含油脂的活饲料生物体研究了不同膳食脂肪酸谱对海豚（Coryphaena hippurus）幼虫存活和亚麻酸（n-3）系列脂肪酸生物转化的影响。第一次喂养（3天）的幼虫要么饥饿，或喂养轮虫（褶皱臂尾轮虫）培养的algaeNannochloropsis oculata和丰富的乳化制剂的玉米，橄榄，大豆和鱿鱼油。对照组投喂N.眼单独。定性脂肪酸组成的甘油三酯（TG）和磷脂（PL）馏分从3日龄至5日龄饥饿的幼虫和7日龄喂养的幼虫进行了测定。此外，以十三烷酸（C13：0）为内标物，测定了7日龄幼虫PL组分中的脂肪酸组成，除了C22：6 n −3的含量在所有幼虫中均发现，但它只存在于富含乳化鱿鱼油的轮虫中。然而，在喂食缺乏这种脂肪酸的轮虫的幼虫中，C22：6 n −3的绝对水平并不高于3日龄的预喂食幼虫。这些幼虫消耗的所有其他脂肪酸的浓度，以及喂食鱿鱼油的幼虫中的C22：6 n −3的浓度，与鱼的干物质重量增加成比例增加。C22：6 n −3和C20：4 n −6被饥饿的幼虫优先保留，尽管脂肪酸含量和类型随着时间的推移而减少。单眼（52%）。然而，生存并没有受到影响的不同的脂肪酸配置文件内的石油丰富groups.It的结论是生物转化的n −3脂肪酸前体的高链C22：6 n −3衍生物不会发生在海豚幼虫至少在他们的第一周的发展。在7日龄幼虫中发现的C22：6 n −3水平似乎来自内源性储存，这些储存一直延续到这一时期。一般来说，C22：6 n −3的这种内源性储备和饮食脂质在支持海豚在早期幼虫期的生存方面都很重要。
The effects of varying dietary fatty acid profile on survival and bioconversion of linolenic (n−3) series fatty acids for dolphin (Coryphaena hippurus) larvae were examined using live feed organisms enriched with oils. First-feeding (3-day-old) larvae were either starved, or fed rotifers (Brachionus plicatilis) cultured on the algaeNannochloropsis oculataand enriched with emulsified preparations of corn, olive, soybean, and squid oils. A control group was fed rotifers packed withN. oculataalone. Qualitative fatty acid composition of triglyceride (TG) and phospholiqid (PL) fractions from 3-day through 5-day old starved larvae and from 7-day-old fed larvae was determined. In addition, quantitative fatty acid content was measured using tridecanoic acid (C13:0) as an internal marker.The fatty acid composition in PL fractions of 7-day-old larvae paralleled that of the particular enriched rotifer they were fed, except for the levels of C22:6n−3 which was found in all larvae, although it was only present in rotifers enriched with the emulsified squid oil. However, the absolute level of C22:6n−3 in larvae fed rotifers deficient in this fatty acid was no higher than it was in 3-day-old, prefeeding larvae. The concentration of all other fatty acids consumed by these larvae, and of C22:6n−3 in larvae fed squid oil, increased in proportion to the dry matter weight gain of the fish. C22:6n−3 and C20:4n−6 were preferentially retained by starved larvae despite a decrease in fatty acid content and type over time.The average survival of all larvae fed oil-enriched rotifers (68%) was 30% greater than that of larvae packed withN. oculataalone (52%). However, survival was not affected by the varying fatty acid profiles of rotifers within the oil-enriched groups.It is concluded that bioconversion ofn−3 fatty acid precursors to the higher chain C22:6n−3 derivative does not occur in dolphin larvae at least during their first week of development. The levels of C22:6n−3 found in 7-day-old larvae appeared to have originated from endogenous stores which were carried over to this period. Both this endogenous reserve of C22:6n−3 and dietary lipids, in general, appear important in supporting survival of dolphins through the early larval period.