A scalable, GFP-based pipeline for membrane protein overexpression screening and purification
A scalable, GFP-based pipeline for membrane protein overexpression screening and purification
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DOI：
10.1110/ps.051466205
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发表时间：
2005-08-01
期刊：
影响因子：
8
通讯作者：
De Gier, JW
中科院分区：
文献类型：
--
作者：
Drew, D;Slotboom, DJ;De Gier, JW
我们描述了一个通用的，基于gfp的管道膜蛋白在大肠杆菌中过表达和纯化。我们举例说明管道的使用大肠杆菌YedZ，一种新的，膜积分黄细胞色素的鉴定和表征。该方法具有可扩展性，适合于高吞吐量应用程序。基于gfp的管道将为大肠杆菌膜蛋白质组的表征提供便利，并为其他膜蛋白质组的表征提供重要参考。
We describe a generic, GFP-based pipeline for membrane protein overexpression and purification in Escherichia coli. We exemplify the use of the pipeline by the identification and characterization of E. coli YedZ, a new, membrane-integral flavocytochrome. The approach is scalable and suitable for high-throughput applications. The GFP-based pipeline will facilitate the characterization of the E. coli membrane proteome and serves as an important reference for the characterization of other membrane proteomes.