Increase in circulating ACE-positive endothelial microparticles during acute lung injury
Increase in circulating ACE-positive endothelial microparticles during acute lung injury
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DOI：
10.1183/13993003.01188-2018
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发表时间：
2019-10
影响因子：
24.3
通讯作者：
Yusuke Takei;M. Yamada;Koji Saito;Yoshinobu Kameyama;H. Sugiura;Tomonori Makiguchi;N. Fujino;A. Koarai;H. Toyama;Kazutomo Saito;Y. Ejima;Yuki Kawazoe;D. Kudo;S. Kushimoto;M. Yamauchi;M. Ichinose
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文献类型：
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作者：
Yusuke Takei;M. Yamada;Koji Saito;Yoshinobu Kameyama;H. Sugiura;Tomonori Makiguchi;N. Fujino;A. Koarai;H. Toyama;Kazutomo Saito;Y. Ejima;Yuki Kawazoe;D. Kudo;S. Kushimoto;M. Yamauchi;M. Ichinose
循环内皮微粒（EMPs）被认为是内皮损伤的标志，肺微血管内皮细胞表达较高水平的血管紧张素转换酶（ACE）。本研究的目的是探讨ACE+微血管emp的数量是否可以作为脓毒症患者急性呼吸窘迫综合征（ARDS）发展的预后指标。用流式细胞术检测人微血管内皮细胞培养上清、小鼠肺损伤模型和脓毒症患者血液中EMPs和ACE+ EMPs的数量。暴露于炎症刺激后，肺微血管内皮细胞培养上清中的ACE+ EMPs增加。在小鼠肺损伤模型中，循环ACE+ EMP和ACE+ EMP/EMP比值均高于对照组（p<0.001）。ACE+ EMP/EMP比值与湿肺/干肺比值相关（rs=0.775, p<0.001）。与未发生ARDS的脓毒症患者相比，脓毒症患者入院时循环ACE+ EMP和ACE+ EMP/EMP比值显著升高（p<0.001）。因此，循环ACE+ EMPs可能是脓毒症患者ARDS发展的预后标志。肺微血管内皮细胞循环ACE+内皮微粒可作为评价肺微血管内皮细胞损伤的标志和脓毒症患者发生ARDS的预后标志http://bit.ly/2GeX7ap
Circulating endothelial microparticles (EMPs) are considered to be markers of endothelial injury, and lung microvascular endothelial cells express higher levels of angiotensin-converting enzyme (ACE). The aim of this study is to examine whether the number of ACE+ microvascular EMPs could be a prognostic marker for the development of acute respiratory distress syndrome (ARDS) in septic patients. The numbers of EMPs and ACE+ EMPs in the culture supernatant from human microvascular endothelial cells, as well as in the blood of mouse lung injury models and septic patients (n=82), were examined using flow cytometry. ACE+ EMPs in the culture supernatant from pulmonary microvascular endothelial cells increased after exposure to an inflammatory stimulus. In the mouse lung injury models, the circulating ACE+ EMPs and ACE+ EMP/EMP ratio were higher than in the controls (p<0.001). The ACE+ EMP/EMP ratio was correlated with the wet/dry lung ratio (rs=0.775, p<0.001). The circulating ACE+ EMPs and ACE+ EMP/EMP ratio on admission were significantly increased in septic patients who developed ARDS compared with septic patients who did not (p<0.001). Therefore, circulating ACE+ EMPs may be a prognostic marker for the development of ARDS in the septic patients. Circulating ACE+ endothelial microparticles from pulmonary microvascular endothelial cells could be a marker for evaluating the injury of pulmonary microvascular endothelial cells and a prognostic marker for developing ARDS in septic patients http://bit.ly/2GeX7ap