Apoptosis of canine mammary tumor cells induced by small interfering RNA (siRNA) against Bcl-xL gene.
Apoptosis of canine mammary tumor cells induced by small interfering RNA (siRNA) against Bcl-xL gene.
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DOI：
10.1292/jvms.68.1199
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发表时间：
2006-11
期刊：
影响因子：
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通讯作者：
F. Tsuchiya;R. Kano;Junichi Sano;K. Oguma;A. Hasegawa
中科院分区：
文献类型：
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作者：
F. Tsuchiya;R. Kano;Junichi Sano;K. Oguma;A. Hasegawa
分析了小干扰RNA (siRNA)对犬乳腺肿瘤细胞系(CF33)中Bcl-xL mRNA表达的抑制和细胞凋亡率的加速。当与siRNA一起培养时，CF33中Bcl-xL转录物的水平降低，表明siRNA可能抑制CF33中Bcl-xL mRNA的表达。当通过 TUNEL 测定评估时，在 Oligofectamine 培养基中添加 siRNA、在 Oligofectamine 培养基中添加双链 RNA、在 Oligofectamine 培养基中添加无 siRNA 以及仅在 DMEM 中培养的 CF33 中，培养 48 小时时的凋亡细胞率分别为 60.9%、30%、28.7% 和 11.6%。这些结果表明犬Bcl-xL可能是犬肿瘤的抗癌靶点。
The inhibition of Bcl-xL mRNA expression and the acceleration of apoptotic cell rates in canine mammary tumor cell line (CF33) by the small interfering RNA (siRNA) were analyzed. The level of Bcl-xL transcripts in CF33 was decreased when cultured with siRNA, suggesting that siRNA might inhibit the expression of Bcl-xL mRNA in the CF33. Apoptotic cell rates in CF33 cultured with siRNA in Oligofectamine medium, with double strand RNA in Oligofectamine medium, without siRNA in Oligofectamine medium and in DMEM alone were 60.9%, 30%, 28.7% and 11.6% at 48-hr incubation, respectively, when evaluated by TUNEL assay. From these results, it was suggested that canine Bcl-xL might be an anticancer target of canine tumors.