Increasing Evidence for and Regulation of a Human Placental Endogenous Digitalis-Like Factor
Increasing Evidence for and Regulation of a Human Placental Endogenous Digitalis-Like Factor
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人胎盘内源性洋地黄样因子的证据和调节不断增加
DOI：
10.1177/1933719111424441
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发表时间：
2012
影响因子：
2.9
通讯作者：
S. Graves
中科院分区：
文献类型：
--
作者：
Jie Ma;M. Esplin;C. Adair;L. Mason;S. Graves
内源性洋地黄样因子（EDLF）似乎是致高血压的，在先兆子痫（PE）妇女的血清和胎盘中增加，先兆子痫是妊娠并发症。Digibind是一种抗地高辛抗体Fab片段，可逆转EDLF的体外作用和PE的体内特征。我们在放射免疫分析中使用Digibind来测量EDLF，并将其与EDLF的生物功能测定进行比较，具有良好的一致性。这些方法证实了人胎盘是EDLF的来源，合成并释放EDLF到培养的人胎盘组织的培养基中。酮康唑，类固醇合成抑制剂，和17-OH孕酮，类固醇合成的可能的底物，显示抑制或增加EDLF释放分别，表明合成途径的重叠。胎盘缺氧、活性氧增加和促炎细胞因子增加等PE的减少可增加胎盘EDLF的释放。这些发现强烈支持胎盘产生EDLF，由于PE的特征而增加释放。
Endogenous digitalis-like factors (EDLFs) appear to be hypertensiogenic and increased in the serum and placenta of women with preeclampsia (PE), a complication of pregnancy. Digibind, an anti-digoxin antibody Fab fragment, reverses in vitro effects of EDLF and in vivo features of PE. We used Digibind in a radioimmunoassay to measure EDLF and compared this to a bio-functional assay of EDLF with good agreement. These methods confirmed that human placenta was a source of EDLF, synthesizing and releasing EDLF into the media of cultured human placental tissue. Ketoconazole, a steroid synthesis inhibitor, and 17-OH progesterone, a possible substrate of steroid synthesis, were shown to inhibit or increase EDLF release respectively, suggesting overlap of synthetic pathways. Abnormalities of PE such as placental hypoxia, increased reactive oxygen species and increased pro-inflammatory cytokines were demonstrated to increase placental EDLF release. These findings strongly support placental production of EDLF with increased release due to features of PE.
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患有先兆子痫、妊娠期短暂性高血压和正常血压妊娠的糖尿病女性中的洋地黄样因子和地高辛样免疫反应因子。
影响因子：
3.2
作者：
Graves,SW;Lincoln,K;Cook,SL;Seely,EW
通讯作者：
Seely,EW
DOI：
10.1016/0002-9378(89)90665-0
发表时间：
1989-11-01
影响因子：
9.8
作者：
ROBERTS, JM;TAYLOR, RN;MCLAUGHLIN, MK
通讯作者：
MCLAUGHLIN, MK
DOI：
--
发表时间：
1986-09
期刊：
The Journal of pharmacology and experimental therapeutics
影响因子：
--
作者：
F. Kraemer;S. Spilman
通讯作者：
F. Kraemer;S. Spilman
影响因子：
3.8
作者：
Li, H;Gu, B;Wang, Y
通讯作者：
Wang, Y
地高辛特异性Fab抗体片段治疗危及生命的洋地黄中毒：26例经验。
DOI：
10.1056/nejm198211253072201
发表时间：
1982
期刊：
The New England journal of medicine
影响因子：
--
作者：
Smith,TW;ButlerJr,VP;Haber,E;Fozzard,H;Marcus,FI;Bremner,WF;Schulman,IC;Phillips,A
通讯作者：
Phillips,A