Spontaneous neutrophil activation in HTLV-1 infected patients
Spontaneous neutrophil activation in HTLV-1 infected patients
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DOI：
10.1590/s1413-86702005000600010
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发表时间：
2005-12-01
影响因子：
3.4
通讯作者：
Carvalho, Edgar M.
中科院分区：
文献类型：
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作者：
Guerreiro, Jaqueline B.;Porto, Maria Aurélia F.;Carvalho, Edgar M.
人类T细胞嗜淋巴病毒1型（HTLV-1）可诱导淋巴细胞活化和增殖，但对HTLV-1感染引起的先天免疫反应知之甚少。我们评估了HTLV-1感染受试者中将硝基蓝四氮唑（NBT）代谢为甲酰胺的中性粒细胞百分比，以及中性粒细胞活化与ifn - γ和tnf - α水平之间的关系。从35名HTLV-1携带者中采集血液，其中8名患有HAM/TSP （HTLV-1相关脊髓病）；对22名健康个体进行自发性和脂多糖（LPS）刺激的中性粒细胞活性（将NBT还原为formazan）评估。ELISA法检测未受刺激的单核细胞产生ifn - γ和tnf - α。自发的NBT水平，以及自发的ifn - γ和tnf - α的产生，显著升高(p
Human T cell lymphotropic Virus type-1 (HTLV-1) induces lymphocyte activation and proliferation, but little is known about the innate immune response due to HTLV-1 infection. We evaluated the percentage of neutrophils that metabolize Nitroblue tetrazolium (NBT) to formazan in HTLV-1 infected subjects and the association between neutrophil activation and IFN-gamma and TNF-alpha levels. Blood was collected from 35 HTLV-1 carriers, from 8 patients with HAM/TSP (HTLV-1- associated myelopathy); 22 healthy individuals were evaluated for spontaneous and lipopolysaccharide (LPS)-stimulated neutrophil activity (reduction of NBT to formazan). The production of IFN-gamma and TNF-alpha by unstimulated mononuclear cells was determined by ELISA. Spontaneous NBT levels, as well as spontaneous IFN-gamma and TNF-alpha production, were significantly higher (p