Optimization of WSSV rVP expression in E. coli cells and minimum dose of rVPs for oral vaccination in kuruma shrimp.
Optimization of WSSV rVP expression in E. coli cells and minimum dose of rVPs for oral vaccination in kuruma shrimp.
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大肠杆菌细胞中 WSSV rVP 表达的优化以及车虾口服疫苗 rVP 的最小剂量。
DOI：
10.3147/jsfp.45.169
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发表时间：
2010
期刊：
影响因子：
0.6
通讯作者：
T. Nishizawa
中科院分区：
文献类型：
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作者：
J. Satoh;H. Kim;T. Matsui;T. Nishizawa
WSSV（= PRDV）rVP 26和rVP 28在E.对IPTG浓度（0.001- 1.0mM）和诱导温度（25 ℃和37 ℃）进行了优化。对于rVP 26和rVP 28，在37°C下在含有0.01 mM IPTG的培养基中观察到最强的表达强度。表达rVP 26或rVP 28的转化菌在含0.01mMIPTG的培养基中的生长与不含IPTG的培养基中的生长几乎相同，但明显延迟于含1.0mMIPTG的培养基。当IPTG浓度从1.0 mM降低到0.01 mM时，rVP 26和rVP 28的生产率分别增加了9倍和6倍。接下来，我们研究了通过实验挑战诱导库鲁马虾对虾对WSD（= PAV）的耐受性所需的rVP的最小剂量。结果表明，至少10 μg/g虾/天的rVP应口服给药15天，以诱导有效的防御。
The optimum conditions for the expression of WSSV (= PRDV) rVP26 and rVP28 in E. coli cells were investigated, especially the IPTG concentration (0.001-1.0 mM) and inducement temperatures (25°C and 37°C). For both rVP26 and rVP28, the strongest intensity of expression was observed in medium containing 0.01 mM IPTG at 37°C. The growth of the transformed bacteria expressing rVP26 or rVP28 in media containing 0.01 mM IPTG was the almost same as that in medium without IPTG, but clearly delayed than that in the medium containing 1.0 mM IPTG. The productivity was increased 9-fold in rVP26 and 6-fold in rVP28 when the IPTG concentration was reduced from 1.0 mM to 0.01 mM. Next, we investigated the minimum doses of rVPs required to induce phylaxis of kuruma shrimp Penaeus japonicus against WSD (= PAV) by an experimental challenge. The results show that at least 10 μg/g shrimp/day of rVPs should be orally administered to kuruma shrimp for 15 days in order to induce effective phylaxis.
口服重组病毒蛋白 rVP26 和 rVP28 的车虾对白斑综合症病毒感染的预防反应的持续时间和增强效果
DOI：
--
发表时间：
2009
期刊：
Fish Pathology 44
影响因子：
--
作者：
Satoh;J.;et al.
通讯作者：
et al.