Caenorhabditis elegans RBX1 is essential for meiosis, mitotic chromosomal condensation and segregation, and cytokinesis
Caenorhabditis elegans RBX1 is essential for meiosis, mitotic chromosomal condensation and segregation, and cytokinesis
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DOI：
10.1046/j.1365-2443.2003.00682.x
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发表时间：
2003-11-01
期刊：
影响因子：
2.1
通讯作者：
Higashitani, A
中科院分区：
文献类型：
--
作者：
Sasagawa, Y;Urano, T;Higashitani, A
背景资料：RING-H2指蛋白RBX 1（ROC 1/HRT 1）是SKP 1-CDC 53/CUL 1-F-box（SCF）、其他cullin和von Hippel-Lindau（VHL）肿瘤抑制因子E3泛素连接酶复合物的共同亚基。RBX 1蛋白序列在各种物种中高度保守，包括酵母、黑腹果蝇、小鼠和人类。在酿酒酵母中，RBX 1是必不可少的G1/S transition.Results：秀丽隐杆线虫RBX 1强烈表达在早期胚胎和性腺，包括减数分裂细胞。通过RNA介导的干扰（RNAi）消耗RBX 1导致第一次减数分裂中的明显缺陷。几个不规则的表型被确定在胚胎中逃脱减数分裂停滞：有丝分裂染色体凝聚和分离，异常染色体桥，巨核，异常皮质突起，多核细胞和生殖细胞增殖缺陷的缺陷。此外，在这些胚胎中，组蛋白H3在Ser（10）和Ser（28）的磷酸化显著降低。结论：RBX 1蛋白参与了染色体代谢和细胞周期调控的多种功能。
Background: The RING-H2 finger protein RBX1 (ROC1/HRT1) is a common subunit of SKP1-CDC53/CUL1-F-box (SCF), other cullins and von Hippel-Lindau (VHL) tumour suppressor E3 ubiquitin ligase complexes. RBX1 protein sequences are highly conserved in various species, including yeasts, Drosophila melanogaster, mice and humans. In Saccharomyces cerevisiae, RBX1 is essential for the G1/S transition.Results: Caenorhabditis elegans RBX1 is strongly expressed in early embryos and in the gonad, including meiotic cells. Depletion of RBX1 by RNA-mediated interference (RNAi) caused pronounced defects in the first meiotic division. Several irregular phenotypes were identified in embryos that escaped from meiotic arrest: defects in mitotic chromosomal condensation and segregation, abnormal chromosome bridges, giant nuclei, abnormal cortical protrusion, multinucleate cells and defects in germ cell proliferation. Moreover, histone H3 phosphorylation at Ser(10) and Ser(28) was significantly reduced in these embryos. The histone H3 phosphorylation defect of embryos was rescued by the additional depletion of protein phosphatase 1 (GLC7alpha/beta) by RNAi.Conclusion: These results indicate that the RBX1 protein participates in diverse functions relevant to chromosome metabolism and cell cycle control.