Force generation by actin polymerization II: The elastic ratchet and tethered filaments
Force generation by actin polymerization II: The elastic ratchet and tethered filaments
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DOI：
10.1016/s0006-3495(03)74969-8
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发表时间：
2003-03-01
影响因子：
3.4
通讯作者：
Oster, G
中科院分区：
文献类型：
--
作者：
Mogilner, A;Oster, G
许多细胞内病原体的运动是由肌动蛋白丝的聚合驱动的。聚合过程开发的推进力被认为是由聚合丝尖的热运动引起的。最近的实验表明，肌动蛋白丝的成核涉及一相，当蛋白质复合物连接到病原体表面时。在这里，我们扩展了Mogilner和Oster的“弹性棘轮模型”，以结合这些新发现。我们应用了这种“绑扎棘轮”模型来得出李斯特菌的力关系关系，并讨论了我们的理论预测与实验测量的关系。我们还讨论了在Acta涂层的珠实验中观察到的“对称性破坏”动力学，以及该模型对迁移细胞中层状脂质突出的含义。
The motion of many intracellular pathogens is driven by the polymerization of actin filaments. The propulsive force developed by the polymerization process is thought to arise from the thermal motions of the polymerizing filament tips. Recent experiments suggest that the nucleation of actin filaments involves a phase when the filaments are attached to the pathogen surface by a protein complex. Here we extend the "elastic ratchet model" of Mogilner and Oster to incorporate these new findings. We apply this "tethered ratchet" model to derive the force-velocity relation for Listeria and discuss relations of our theoretical predictions to experimental measurements. We also discuss "symmetry breaking" dynamics observed in ActA-coated bead experiments, and the implications of the model for lamellipodial protrusion in migrating cells.