The chemical structure and genetic locus of Campylobacter jejuni CG8486 (serotype HS:4) capsular polysaccharide:: the identification of 6-deoxy-D-ido-heptopyranose
The chemical structure and genetic locus of Campylobacter jejuni CG8486 (serotype HS:4) capsular polysaccharide:: the identification of 6-deoxy-D-ido-heptopyranose
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DOI：
10.1016/j.carres.2008.02.024
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发表时间：
2008-05-05
影响因子：
3.1
通讯作者：
Monteiro, Mario A.
中科院分区：
文献类型：
--
作者：
Chen, Yu-Han;Poly, Frederic;Monteiro, Mario A.
根据我们基于空肠弯曲杆菌胶囊多糖（CPS）的多价抗空肠弯曲杆菌疫苗的持续努力，我们在这里报告了空肠弯曲杆菌菌株CG8486产生的CPS的化学结构和遗传位点，该菌株属于血清型HS:4 CPS复合物。空肠C. CG8486 CPS由大约17个4-取代n -乙酰- β -d -葡萄糖氨基和3-取代6-脱氧- β -d -ido-庚基氨基的双糖重复块组成。少量的6-脱氧- β -d -ido-庚吡喃糖单元在O-2或O-7位置携带o-甲基磷酰胺基团。空肠C. CG8486 CPS位点的基因含量和结构与NCTC 11168和81-176相似，但有几个CPS基因与NCTC 11168和81-176 CPS位点的基因差异更大，说明空肠C. HS:4 CPS复合物具有特异性的遗传特征。基于空肠C. CG8486 CPS的糖结合疫苗的功效目前正在动物模型中进行测试，其结果将在今后的通讯中提出。(c) 2008 Elsevier Ltd.版权所有。
In line with our on-going efforts to create a multivalent anti-Campylobacter jejuni vaccine based on its capsule polysaccharides (CPSs), we report here the chemical structure and the genetic locus of the CPS produced by C. jejuni strain CG8486, which belongs to the serotype HS:4 CPS complex. C. jejuni CG8486 CPS was observed to be composed of approximately 17 disaccharide repeating blocks of 4-substituted N-acetyl-beta-D-glucopyranosamine and 3-substituted 6-deoxy-beta-D-ido-heptopyranose. A small number of 6-deoxy-beta-D-ido-heptopyranose units were observed to carry O-methyl phosphoramidate moieties at the O-2 or O-7 position. The gene content and organization of the CPS locus of C. jejuni CG8486 were comparable to those of C jejuni strains NCTC 11168 and 81-176, but several CG8486 CPS genes were observed to be more divergent from those present in the CPS loci of NCTC 11168 and 81-176 CPS, which indicated that there are genetic characteristics specific to the C. jejuni HS:4 CPS complex. The efficacy of a glycoconjugate vaccine based on C. jejuni CG8486 CPS is presently being tested in an animal model, the results of which will be presented in future communications. (c) 2008 Elsevier Ltd. All rights reserved.