Oxidized BAL fluid proteins in patients with interstitial lung diseases
Oxidized BAL fluid proteins in patients with interstitial lung diseases
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DOI：
10.1183/09031936.96.09020307
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发表时间：
1996-02-01
影响因子：
24.3
通讯作者：
Maier, KL
中科院分区：
文献类型：
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作者：
Lenz, AG;Costabel, U;Maier, KL
由吞噬细胞释放的氧源性自由基被认为是肺组织损伤的原因。因此，我们研究了支气管肺泡灌洗液（BALF）中蛋白质的氧化损伤，作为氧化应激的指标，并评估肺中的抗氧化防御。我们检查了间质性肺病患者的BALF液，如特发性肺纤维化（IPF，非吸烟者（NS）和吸烟者（S）），结节病（SARC，非吸烟者）和石棉肺（ASB，戒烟者（EXS））。BALF蛋白质的氧化伴随着羰基引入到它们的氨基酸侧链中，并且可以通过用氚化硼氢化物标记这些基团来定量。NS对照组（n=9）BALF（平均值+/-SEM）mL，无症状S组（m=8; 0.59+/-0.14 nmol . mL（-1））。该参数在IPF-NS中显著升高（n=14; 0.84+/-0.2 nmol羰基。mL（-1）BALF）和SARC-NS（n=15; 0.73+/-0.16 nmol . mL（-1））。相反，在吸烟的IPF患者中（n=6; 0.41+/-0.1 nmol羰基。mL（-1）BALF）和ASB-EXS（n=6; 0.73+/-0.16 nmol . mL（-1）），与NS对照组无显著性差异。氧化蛋白总量与IPF-NS、IPF-S和SARC中嗜酸性粒细胞（EOS）的绝对数量呈正相关，也与IPF-NS和IPF-S中中性粒细胞（PMN）的绝对数量呈正相关。总之，非吸烟IPF和SARC患者的BALF蛋白发生氧化损伤。氧化支气管肺泡灌洗液蛋白的量可以提供氧负荷的定量评估，氧化应激和抗氧化防御之间的平衡。
Oxygen-derived free radicals, released by phagocytic cells, have been postulated to contribute to lung tissue damage. We therefore investigated oxidative damage to proteins from bronchoalveolar lavage fluid (BALF) as an indicator of oxidative stress and to assess antioxidant defences in the lungs.We examined BAL fluids from patients with interstitial lung diseases, such as idiopathic pulmonary fibrosis (IPF, nonsmokers (NS) and smokers (S)), sarcoidosis (SARC, nonsmokers), and asbestosis (ASB, ex-smokers (EXS)). The oxidation of BALF proteins is accompanied by the introduction of carbonyl groups into their amino acid side-chains and can be quantitated by labelling these groups with tritiated borohydride.The total lung content of oxidized proteins recovered by bronchoalveolar lavage (BAL) was 0.3+/-0.07 nmol carbonyl . mL BALF (mean+/-SEM) in the NS control group (n=9) and tended to be increased, in the asymptomatic S group (m=8; 0.59+/-0.14 nmol . mL(-1)). This parameter was significantly elevated both in IPF-NS (n=14; 0.84+/-0.2 nmol carbonyl . mL(-1) BALF) and SARC-NS (n=15; 0.73+/-0.16 nmol . mL(-1)) as compared with NS control. On the contrary, in smoking patients with IPF (n=6; 0.41+/-0.1 nmol carbonyl . mL(-1) BALF) and also in ASB-EXS (n=6; 0.73+/-0.16 nmol . mL(-1)) it was not different from NS controls. The total amount of oxidized proteins correlated positively with the absolute number of eosinophils (EOS) in IPF-NS, IPF-S and SARC and also with absolute polymorphonuclear neutrophil (PMN) numbers in IPF-NS and IPF-S.In conclusion, oxidative damage of BALF proteins occurred in nonsmoking patients with IPF and SARC. The amount of oxidized bronchoalveolar lavage fluid protein may provide a quantitative assessment of oxygen burden, a balance between oxidant stress and antioxidant defences.