Rapid purification of cell encapsulated hydrogel beads from oil phase to aqueous phase in a microfluidic device
Rapid purification of cell encapsulated hydrogel beads from oil phase to aqueous phase in a microfluidic device
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在微流体装置中快速纯化细胞封装的水凝胶珠从油相到水相
DOI：
10.1039/c1lc20494g
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发表时间：
2011-01-01
期刊：
影响因子：
6.1
通讯作者：
Zhao, Xing-Zhong
中科院分区：
文献类型：
--
作者：
Deng, Yuliang;Zhang, Nangang;Zhao, Xing-Zhong
在本文中，我们展示了一种新型的微流控芯片，可以实现连续流纯化的水凝胶珠从载体油到水溶液通过使用层状油/水界面。微流控芯片由两个功能组件组成：（1）可以控制珠粒大小和形状的流动聚焦珠粒生成模块，（2）能够将水凝胶珠粒从油中纯化成水溶液的珠粒提取模块。该模块的特点是一侧有大分支通道，另一侧有小通道。水通过大的分支通道连续地注入珠提取模块中，从而在分支接合部之间产生层状油/水界面。模拟和实验数据表明，驱油效率取决于注入水和载体油之间的相对流量。通过使用这种微流体装置，活细胞（HCT 116，结肠癌细胞系）可以包封在水凝胶珠中并纯化到细胞培养基中。与通过常规珠纯化方法观察到的细胞活力相比，实现了显著改善的细胞活力。这种简单的微流控芯片可能是一个有前途的候选人在芯片实验室应用中的样品处理。
In this paper, we demonstrate a new type of microfluidic chip that can realize continuous-flow purification of hydrogel beads from a carrier oil into aqueous solution by using a laminar-like oil/water interface. The microfluidic chip is composed by two functional components: (1) a flow-focusing bead generation module that can control size and shape of beads, (2) a bead extraction module capable of purifying hydrogel beads from oil into aqueous solution. This module is featured with large branch channels on one side and small ones on the opposite side. Water is continuously infused into the bead extraction module through the large branch channels, resulting in a laminar-like oil/water interface between the branch junctions. Simulation and experimental data show that the efficiency of oil depletion is determined by the relative flow rates between infused water and carrier oil. By using such a microfluidic device, viable cells (HCT116, colon cancer cell line) can be encapsulated in the hydrogel beads and purified into a cell culture media. Significantly improved cell viability was achieved compared to that observed by conventional bead purification approaches. This facile microfluidic chip could be a promising candidate for sample treatment in lab-on-a-chip applications.