Elevated expressions of serum miR-15a, miR-16, and miR-17-5p are associated with acute ischemic stroke.
Elevated expressions of serum miR-15a, miR-16, and miR-17-5p are associated with acute ischemic stroke.
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血清 miR-15a、miR-16 和 miR-17-5p 表达升高与急性缺血性中风相关。
DOI：
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发表时间：
2015-11
期刊：
影响因子：
--
通讯作者：
Lu, Xiang
中科院分区：
文献类型：
--
作者：
Wu, Jin;Du, Kang;Lu, Xiang
急性缺血性中风（AIS）是世界范围内导致死亡和残疾的主要原因之一。循环microRNAs （miRNAs）已被确定为包括AIS在内的多种人类疾病的诊断和预后的潜在生物标志物。本研究采用实时定量聚合酶链式反应（qRT-PCR）检测了AIS患者（n = 106）和健康对照（n = 120）血清中miR-15a、miR-16和miR-17-5p的表达水平。进行统计学分析以调查miRNA水平与AIS风险之间的关系。与对照组相比，AIS患者血清中miR-15a、miR-16和miR-17-5p的表达水平分别升高8.3倍（P = 0.0104）、42倍（P < 0.0001）和9.9倍（P = 0.0002）。多因素logistic回归显示血清miR-17-5p水平是确定AIS存在的重要且独立的预测因子。受试者工作特征（ROC）分析显示，miR-15a、miR-16和miR-17-5p的曲线下面积（AUC）分别为0.698（95%可信区间[CI]: 0.559-0.837, P = 0.01）、0.82 （95% CI: 0.71-0.931, P < 0.001）和0.784 (95% CI: 0.666-0.903, P < 0.001)，而这三种micorna组合的AUC增加到0.845 （95% CI: 0.74-0.949, P < 0.001）。我们的研究结果表明，miR-15a、miR-16和miR-17-5p的血清表达升高与AIS密切相关，这三种microrna的组合可能是AIS的一种有希望的血清生物标志物。
Acute ischemic stroke (AIS) is one of the leading causes of death and disability worldwide. Circulating microRNAs (miRNAs) have been identified as potential biomarkers in the diagnosis and prognosis of multiple human diseases including AIS. In this study, serum expression levels of miR-15a, miR-16, and miR-17-5p were detected in AIS patients (n = 106) and healthy controls (n = 120) using quantitative real-time polymerase chain reaction (qRT-PCR). Statistical analyses were performed to investigate the associations between miRNA levels and AIS risk. The serum expression levels of miR-15a, miR-16, and miR-17-5p increased by 8.3 fold (P = 0.0104), 42 fold (P < 0.0001), and 9.9 fold (P = 0.0002) in AIS patients compared to controls, respectively. Multivariate logistic regression demonstrated that serum miR-17-5p level was a significant and independent predictor for determining the presence of AIS. Receiver operating characteristic (ROC) analysis revealed areas under the curves (AUCs) of 0.698 (95% confidence interval [CI]: 0.559-0.837, P = 0.01), 0.82 (95% CI: 0.71-0.931, P < 0.001), and 0.784 (95% CI: 0.666-0.903, P < 0.001) for miR-15a, miR-16, and miR-17-5p, respectively, while the AUC increased to 0.845 (95% CI: 0.74-0.949, P < 0.001) for the combination of these three micoRNAs. Our findings indicate that elevated serum expression of miR-15a, miR-16, and miR-17-5p is strongly associated with AIS and that the combination of these three microRNAs may be a promising serum biomarker for AIS.
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DOI：
10.3724/sp.j.1263.2012.00028
发表时间：
2012-03
期刊：
Journal of geriatric cardiology : JGC
影响因子：
--
作者：
Liu LF;Liang Z;Lv ZR;Liu XH;Bai J;Chen J;Chen C;Wang Y
通讯作者：
Wang Y
影响因子：
168.9
作者：
Lozano, Rafael;Naghavi, Mohsen;Murray, Christopher J. L.
通讯作者：
Murray, Christopher J. L.
DOI：
10.1007/978-1-4939-7435-1
发表时间：
2018
期刊：
--
影响因子：
--
作者：
Wei Wu
通讯作者：
Wei Wu
DOI：
10.1007/978-1-60761-863-8
发表时间：
2011
期刊：
--
影响因子：
--
作者：
Wei Wu
通讯作者：
Wei Wu
影响因子：
82.9
作者：
Bonci, Desiree;Coppola, Valeria;De Maria, Ruggero
通讯作者：
De Maria, Ruggero