Geographic distribution of vacA allelic types of Helicobacter pylori
Geographic distribution of vacA allelic types of Helicobacter pylori
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DOI：
10.1016/s0016-5085(99)70065-x
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发表时间：
1999-04-01
期刊：
影响因子：
29.4
通讯作者：
Queiroz, DMD
中科院分区：
文献类型：
--
作者：
van Doorn, LJ;Figueiredo, C;Queiroz, DMD
背景和目标：幽门螺杆菌vacA的不同等位基因类型已被确定，本研究评估了vacA等位基因和cagA的地理分布。研究方法：采用聚合酶链反应和线性探针反向杂交法（利帕）对来自24个国家的735例患者的培养物进行了分析。结果：在735例培养物中，有124例（16.9%）检出了多个vacA基因型，可对611株菌株进行分析。在欧洲，观察到sl亚型的分布梯度。在北方和东欧，89%是s1 b亚型。s1 a和s1 b在法国和意大利同样存在，而在西班牙和葡萄牙，89%的菌株为s1 b亚型。sla和s1 b在北美的流行率大致相等，在中美洲和南美洲，几乎所有的s1毒株都是s1 b亚型。在东亚的sl分离株中观察到77%的s1 c亚型。除了伊比利亚半岛和中美洲和南美洲，m1（86.2%）比m2（13.8%）更普遍外，m1和m2 a具有同等的存在。亚型m2 b仅见于东亚s1 c株。vacAs 1/cagA阳性基因型与消化性溃疡的发病有关（P < 0.001）。pylori基因型，有助于理解H. pylori疾病
Background & Aims: Distinct allelic types of Helicobacter pylori vacA have been defined, The geographic distribution of vacA alleles and cagA was assessed in this study. Methods: A total of 735 cultures from patients in 24 countries were analyzed by polymerase chain reaction and reverse hybridization on a line probe assay (LiPA), Results: In 124 (16.9%) of the 735 cultures, multiple vacA genotypes were detected, permitting analysis of 611 strains. In Europe, a distribution gradient of sl subtypes was observed. In northern and eastern Europe, 89% were subtype s1b. s1a and s1b were equally present in France and Italy, whereas in Spain and Portugal 89% of strains were subtype s1b. sla and s1b were approximately equally prevalent in North America, In Central and South America, virtually all s1 strains were subtype s1b. Subtype s1c was observed in 77% of the sl isolates from East Asia. m1 and m2a have equal presence, except on the Iberian peninsula and in Central and South America, where m1 (86.2%) is more prevalent than m2 (13.8%). Subtype m2b was found exclusively among East Asian s1c strains. In all parts of the world, vacA s1/cagA-positive genotypes were associated with peptic ulcer disease (P < 0.001), Conclusions: These data indicate a geographic distribution of H. pylori genotypes and aid in understanding the relationship of H. pylori with disease.