Steady-state multispectral fluorescence imaging system for plant leaves
Steady-state multispectral fluorescence imaging system for plant leaves
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DOI：
10.1364/ao.40.000157
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发表时间：
2001-01-01
期刊：
影响因子：
1.9
通讯作者：
Chen, YR
中科院分区：
文献类型：
--
作者：
Kim, MS;McMurtrey, JE;Chen, YR
我们提出了一个基于实验室的植物叶片多光谱荧光成像系统(MFIS)的详细描述。在360 nm激发下，在蓝、绿、红和远红四个光谱带分别以450、550、680和740 nm为中心捕获了荧光发射。用除草剂(DCMU)处理的大豆叶片和短期暴露在中等升高的对流层臭氧环境中进行的初步实验证明了新开发的微型燃料电池的效用。此外，借助正常大豆叶片的荧光图像，讨论了影响荧光发射的几种机制。成像结果说明了荧光成像的多功能性，它提供了关于叶片样品上荧光模式的空间变异性的信息。OCS码：170.0110、170.6280、300.2140、300.2530、040.1520。
We present a detailed description of a laboratory-based multispectral fluorescence imaging system (MFIS) for plant leaves. Fluorescence emissions with 360-nm excitation are captured at four spectral bands in the blue, green, red, and far-red regions of the spectrum centered at 450, 550, 680, and 740 nm, respectively. Preliminary experiments conducted with soybean leaves treated with a herbicide (DCMU) and short-term exposures to moderately elevated tropospheric ozone environment demonstrated the utilities of the newly developed MFIS. In addition, with the aid of fluorescence images of normal soybean leaves, several mechanisms governing the fluorescence emissions are discussed. Imaging results illustrate the versatility of fluorescence imaging, which provides information on the spatial variability of fluorescence patterns over leaf samples. OCIS codes: 170.0110, 170.6280, 300.2140, 300.2530, 040.1520.