Using synergists to detect multiple insecticide resistance in field populations of rice stem borer
Using synergists to detect multiple insecticide resistance in field populations of rice stem borer
复制标题
使用增效剂检测稻螟田间种群对多种杀虫剂的抗药性
DOI：
10.1016/j.pestbp.2012.04.008
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发表时间：
2012-06-01
影响因子：
4.7
通讯作者：
Shen, Jinliang
中科院分区：
文献类型：
--
作者：
He, Yueping;Zhang, Juefeng;Shen, Jinliang
水稻二化螟Chilo suppressalis（步行者）是我国水稻的重要害虫，在田间已对多种杀虫剂产生抗药性。为探讨小菜蛾的多重抗性机制，以瑞安种群和连云港种群为试材，进行了对不同杀虫剂的增效试验。结果表明，顺丁烯二酸二乙酯（DEM）、S，S，S-三丁基三硫代磷酸酯（DEF）和胡椒基丁醚（PBO）对2011年（RA 11）以来瑞安市田间种群对杀虫单高抗（313.4倍）和毒死蜱中抗（36.9倍）均无明显增效或抑制作用。DEF对三唑磷在RA 11种群中的增效作用达536.8倍，增效比为1.92。DEF和PBO分别显著抑制了RA 11种群对氟虫腈耐药性的43.3%和40.4%（48.4倍），协同比分别为1.76和1.69。PBO预处理对溴氰菊酯的增效作用显著，增效比为9.57，抗性水平降低152.5 ~ 15.9倍。研究结果表明，田间种群对几种杀虫剂的抗性程度不同。抑制可能是由多种抗性机制的组合提供的。代谢抗性机制可能是导致对溴氰菊酯和氟虫腈抗性进化的主要原因，而对杀虫单、三唑磷和毒死蜱的抗性与代谢抗性无关。(C)2012年由Elsevier Inc.出版
The rice stem borer, Chilo suppressalis (Walker), an important insect pest of rice in China, has developed resistances to several classes of insecticides in field. In order to investigate multiple resistance mechanisms, synergistic tests were conducted with the Ruian (RA) population and Lianyungang (LYG) population, two representative populations to different insecticides. Results showed a at diethyl maleate (DEM), S,S,S-tributyl phosphorotrithioate (DEF) and piperonyl butoxide (PBO), had no significant synergistic or inhibitory effect on the high level of resistance to monosultap (313.4-fold) and moderate level to chlorpyrifos (36.9-fold) in Ruian field population from the year 2011 (RA11). DEF significantly synergized the activity of triazophos in RA11 population (536.8-fold), with synergism ratio of 1.92. DEF and PBO significantly suppressed 43.3% and 40.4% of resistance to fipronil in RA11 population (48.4-fold), respectively, with the synergistic ratios of 1.76 and 1.69. When pretreated with PBO, the activity of deltamethrin against RA11 population were significantly synergized, with synergism ratio of 9.57, and with reducing resistance levels from 152.5- to 15.9-fold. The results of this study indicated that resistance to several classes of insecticide among the field populations of C. suppressalis might be provided by the combination of the multiple resistance mechanisms. Metabolic resistance mechanism might be the major reason for the evolution for resistance to deltamethrin and fipronil, while resistance to monosultap, triazophos and chlorpyrifos is not associated with metabolic resistance. (C) 2012 Published by Elsevier Inc.