Variation in Vibrio parahaemolyticus isolates from a single Thai shrimp farm experiencing an outbreak of acute hepatopancreatic necrosis disease (AHPND)
Variation in Vibrio parahaemolyticus isolates from a single Thai shrimp farm experiencing an outbreak of acute hepatopancreatic necrosis disease (AHPND)
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DOI：
10.1016/j.aquaculture.2014.03.030
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发表时间：
2014-05-20
期刊：
影响因子：
4.5
通讯作者：
Thitamadee, Siripong
中科院分区：
文献类型：
--
作者：
Joshi, Jyoti;Srisala, Jiraporn;Thitamadee, Siripong
2013年泰国渔业部（DOF）估计，养殖虾中急性早期死亡（通常称为早期死亡综合征或EMS）的爆发是导致2013年第一季度虾产量下降33%的原因。越南类似的早期死亡归因于引起急性肝胰腺坏死病（AHPND）的副溶血性弧菌的特定分离株，但泰国的EMS/AHPND状况尚不清楚。在这里，我们描述了从泰国一个早期死亡爆发农场收集的虾肝胰脏（HP）中分离出的细菌的分离和特性。通过BLAST分析和卵磷脂依赖性溶血素（LDH）的基因特异性标记检测，鉴定出4株独立的细菌分离株为副溶血性弧菌。其中3种和参考分离物（2010年从中国获得）使用先前发表的实验室感染模型进行浸泡挑战，导致虾HP的死亡率非常高，并伴有典型的AHPND组织病理学。对其中一个分离株（5HP）的试验表明，死亡率与剂量有关。采用相同的攻毒方案，第4株分离株（2HP）也造成了高死亡率，但没有伴有AHPND的组织病理学。相反，它引起HP的不同组织病理学，包括上皮塌陷和胚胎细胞（e细胞）独特的空泡化。这些结果揭示了副溶血性弧菌可能存在多样性，并可能导致对虾养殖池的早期死亡。需要对这些分离株和不引起疾病的副溶血性弧菌的分离株的基因组和表观DNA进行比较，以更好地了解AHPND细菌毒力的分子基础。(C) 2014 Elsevier B.V.版权所有
The Thai Department of Fisheries (DOF), 2013 estimated that outbreaks of acute early mortality (often called early mortality syndrome or EMS) in cultivated shrimp were responsible for a 33% drop in shrimp production during the first quarter of 2013. Similar early mortality in Vietnam was ascribed to specific isolates of Vibrio parahaemolyticus that caused acute hepatopancreatic necrosis disease (AHPND) but the status of EMS/AHPND in Thailand was unclear. Here we describe the isolation and characterization of bacteria isolated from the hepatopancreas (HP) of shrimp collected from an early mortality outbreak farm in Thailand. Four independent bacterial isolates were identified as V. parahaemolyticus by BLAST analysis and by gene-specific marker detection of a lecithin dependent hemolysin (LDH) considered to be specific for the species. Immersion challenges with 3 of these and a reference isolate, obtained from China in 2010, using a previously published laboratory infection model caused very high mortality accompanied by characteristic AHPND histopathology in the shrimp HP. Tests with one of these isolates (5HP) revealed that rate of mortality was dose dependent. Using the same challenge protocol, the 4th isolate (2HP) also caused high mortality, but it was not accompanied by AHPND histopathology. Instead, it caused a different histopathology of the HP including collapsed epithelia and unique vacuolization of embryonic cells (E-cells). These results revealed the possibility of diversity in isolates of V. parahaemolyticus that may cause early mortality in shrimp cultivation ponds. Genomic and episomic DNA of these isolates and isolates of V. parahaemolyticus that cause no disease need to be compared to better understand the molecular basis of bacterial virulence in AHPND. (C) 2014 Elsevier B.V. All rights reserved.