Perfusion fixation of rat molar pulp tissue for light microscopy.
Perfusion fixation of rat molar pulp tissue for light microscopy.
复制标题
光学显微镜下大鼠磨牙牙髓组织的灌注固定。
DOI：
10.1111/j.1600-0722.1973.tb01504.x
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发表时间：
1973
期刊：
影响因子：
--
通讯作者：
Synnöve Heide
中科院分区：
文献类型：
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作者：
Synnöve Heide
– 对 42 只大鼠的磨牙牙髓组织进行心脏灌注固定或用戊二醛和甲醛浸泡固定。研究了心脏灌注压力和持续时间变化的重要性。结果表明，灌注法优于浸泡固定。当在 130 cmH2O 压力下用 1.7% 戊二醛灌注 10-12 分钟，并将动物放置 4 小时（不进行浸没固定）时，可获得最佳的组织保存效果。如果溶液是由多聚甲醛粉末制备的，则用甲醛灌注也能获得良好的保存效果。相比之下，由商业甲醛储备溶液制成的溶液结果较差。对于用苏木精-伊红染色的切片，优选用戊二醛灌注，而用甲醛灌注在显示用pH 3.6的阿新蓝染色的牙本质小管时显示出更高的敏感性。建议今后对大鼠牙髓组织的研究应采用戊二醛和甲醛灌注代替浸泡固定。
– Molar pulp tissue of 42 rats was subjected to cardiac perfusion fixation or to immersion fixation with glutaraldehyde and formaldehyde. The importance of variations in pressure and duration of cardiac perfusion was studied. The results indicated that the perfusion method was superior to immersion fixation. Best preservation of the tissues was obtained when perfusion was performed with 1.7% glutaraldehyde for 10–12 min under a pressure of 130 cmH2O, and leaving the animals for 4 h, without immersion fixation. Also perfusion with formaldehyde resulted in good preservation, provided the solutions were prepared from paraformaldehyde powder. In contrast, solutions made from commercial stock solutions of formaldehyde gave inferior results. For sections stained with hematoxylin-eosin, perfusion with glutaraldehyde was preferable, while perfusion with formaldehyde showed increased sensitivity in demonstration of dentinal tubules stained with alcian blue at pH 3.6. It is suggested that in future studies on rat pulp tissue, perfusion with glutaraldehyde and formaldehyde should replace immersion fixation.