Genetic enhancement of lodging resistance in rice due to the key cell wall polymer lignin, which affects stem characteristics
Genetic enhancement of lodging resistance in rice due to the key cell wall polymer lignin, which affects stem characteristics
复制标题
由于关键细胞壁聚合物木质素影响茎特性，水稻抗倒伏性得到遗传增强
DOI：
10.1270/jsbbs.18050
复制
发表时间：
2018-12-01
期刊：
影响因子：
2.4
通讯作者：
Li, Fengcheng
中科院分区：
文献类型：
--
作者：
Liu, Sitong;Huang, Yuwei;Li, Fengcheng
农作物倒伏严重制约植物生长和粮食生产。对细胞壁进行遗传修饰以增强植物的机械强度已被认为是提高植物抗倒伏能力的一个有希望的途径。然而，由于植物细胞壁的复杂性，其聚合物对植物抗倒伏的确切作用尚不清楚。为了解决这一问题，我们对56个表现出不同细胞壁成分和倒伏指数的水稻品种进行了大规模分析。木质素是决定水稻抗倒伏性的关键细胞壁聚合物。细胞壁组成与植株形态特征的相关分析表明，木质素通过大幅度提高基茎机械强度和降低株高来增强水稻抗倒伏能力。通过对4个具有不同抗倒伏能力的代表性水稻品种神农9903、盐健218、空育131和神农k33的进一步鉴定，揭示了木质素水平升高的多个候选基因（PAL、CoMT、4CL3、CAD2、CAD7和CCR20）。因此。我们的研究结果表明，细胞壁中高水平的木质素显著提高了水稻的抗倒伏能力，并为培育高抗倒伏水稻提供了木质素基因改造的靶基因。
Lodging in crops seriously restricts plant growth and grain production. The genetic modification of cell walls to enhance plant mechanical strength has been suggested as a promising approach toward improving lodging resistance. However, because of the complexity of the plant cell wall, the exact effects of its polymers on plant lodging resistance remain elusive. To address this issue, we performed large-scale analyses of a total of 56 rice (Oryza sativa L.) varieties that displayed distinct cell wall component and lodging index. Lignin was identified as the key cell wall polymer that positively determines lodging resistance in rice. Correlation analysis between cell wall composition and plant morphological characteristics revealed that lignin enhanced rice lodging resistance by largely increasing the mechanical strength of the basal stem and reducing plant height. Further characterization of four representative rice varieties, ShenNong9903, YanJian218, KongYu131, and ShenNongK33, displaying varied levels of lodging resistance, revealed the multiple candidate genes (PAL, CoMT, 4CL3, CAD2, CAD7 and CCR20) responsible for increasing lignin level. Hence. our results demonstrate that the high lignin level in the cell wall predominately improves lodging resistance and suggest target genes for the genetic modification of lignin towards breeding rice with high lodging resistance.