DETERMINATION OF MICS FOR MYCOBACTERIUM-AVIUM MYCOBACTERIUM-INTRACELLULARE COMPLEX IN LIQUID-MEDIUM BY A COLORIMETRIC METHOD
DETERMINATION OF MICS FOR MYCOBACTERIUM-AVIUM MYCOBACTERIUM-INTRACELLULARE COMPLEX IN LIQUID-MEDIUM BY A COLORIMETRIC METHOD
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DOI：
10.1128/jcm.33.7.1842-1846.1995
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发表时间：
1995-07-01
影响因子：
9.4
通讯作者：
MEHTA, RT
中科院分区：
文献类型：
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作者：
GOMEZFLORES, R;GUPTA, S;MEHTA, RT
我们研究了基于二甲噻唑-二苯基溴化四唑（MTT）还原的快速比色微法测定鸟分枝杆菌m生长的潜力。氯法齐明intracellulare复杂(MAC)和中等收入国家,resorcinomycin,对MAC和喹诺酮PD 127391。麻省理工的减少直接与可行的细菌的数量成正比。MIT还原试验与[H-3]甘油摄取试验的比较表明，前者在检测微量滴度板中MAC生长方面具有更高的分析灵敏度。MTT还原试验避免使用放射性同位素和昂贵的材料和设备；该方法可靠、可重复性好、便于快速常规药敏试验。
We investigated the potential of a rapid colorimetric microassay based on the reduction of dimethylthiazol-diphenyltetrazolium bromide (MTT) for determining the growth of Mycobacterium avium-M. intracellulare complex (MAC) and MICs of clofazimine, resorcinomycin A, and the quinolone PD 127391 against MAC. The reduction of MTT was directly proportional to the number of viable bacteria. A comparison of the MIT reduction test with the [H-3] glycerol uptake assay show ed the former to possess higher analytical sensitivity for detecting MAC growth in microtiter plates. The MTT reduction test avoids the use of radioisotopes and costly material and equipment; it is reliable, reproducible, and convenient for rapid routine susceptibility testing of MAC.