SALMONELLA-TYPHIMURIUM PHOP VIRULENCE GENE IS A TRANSCRIPTIONAL REGULATOR
SALMONELLA-TYPHIMURIUM PHOP VIRULENCE GENE IS A TRANSCRIPTIONAL REGULATOR
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DOI：
10.1073/pnas.86.18.7077
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发表时间：
1989-09-01
影响因子：
11.1
通讯作者：
HEFFRON, F
中科院分区：
文献类型：
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作者：
GROISMAN, EA;CHIAO, E;HEFFRON, F
鼠伤寒沙门氏菌是一种兼性胞内病原体，能在宿主吞噬细胞内存活。携带Phop突变的沙门氏菌株是无毒的，不能在巨噬细胞中生存，并且对具有抗菌活性的多肽非常敏感，例如宿主衍生的防御素。我们给出了Phop基因的DNA序列，并表明Phop的推导氨基酸序列与大肠杆菌转录调控因子Phob和OmpR具有广泛的同源性，后者控制着基因座对不同环境刺激的反应。PSID基因受磷酸盐供应的调节，受Phop基因产物的控制。在几种革兰氏阴性菌和酿酒酵母中发现了与Phop同源的序列。
Salmonella typhimurium is a facultative intracellular pathogen capable of surviving within host phagocytic cells. Salmonella strains carrying phoP mutations are avirulent, unable to survive in macrophages, and extremely sensitive to peptides having antimicrobial activity such as the host-derived defensins. We present here the DNA sequence of the phoP gene and show that the deduced amino acid sequence of phoP has extensive homology with the Escherichia coli transcriptional regulators PhoB and OmpR, which control the expression of loci in response to different environmental stimuli. The psiD locus, which is regulated by phosphate availability, was found to be under the control of the phoP gene product. Sequences homologous to phoP were found in several Gram-negative species and in the yeast Saccharomyces cerevisiae.