Cytochrome c-specific protein methylase III from Neurospora crassa.
Cytochrome c-specific protein methylase III from Neurospora crassa.
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来自粗糙脉孢菌的细胞色素 c 特异性蛋白甲基化酶 III。
DOI：
10.1042/bj1650011
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发表时间：
1977
期刊：
影响因子：
--
通讯作者：
W. Paik
中科院分区：
文献类型：
--
作者：
S. Nochumson;E. Durban;S. Kim;W. Paik
一种蛋白质甲基化酶III负责具体甲基化的细胞色素c在粗糙脉孢菌的部分特征在于使用未甲基化的马心细胞色素c作为底物。该酶利用S-腺苷-L-甲硫氨酸作为甲基供体。通过胰凝乳蛋白酶消化酶促甲基化细胞色素c获得的肽中[14 C]甲基的分布分析表明，色谱后所有放射性均可在单个峰内回收。这表明该酶甲基化细胞色素c内的特定氨基酸序列。在水解放射性胰凝乳蛋白酶肽时，鉴定了Me-14 C标记的ε-N-单甲基-赖氨酸、ε-N-二甲基-赖氨酸和ε-N-三甲基-赖氨酸。该酶可以很容易地从N. crassa菌丝垫，并纯化约。30倍
A protein methylase III responsible for specifically methylating the cytochrome c in Neurospora crassa was partially characterized by using unmethylated horse heart cytochrome c as a substrate. This enzyme utilizes S-adenosyl-L-methionine as the methyl donor. An analysis of the distribution of [14C]methyl groups in the peptides obtained by chymotrypsin digestion of the enzymically methylated cytochrome c showed that all of the radioactivity could be recovered within a single peak after chromatography. This indicates that the enzyme methylates a specific amino acid sequence within cytochrome c. On hydrolysis of the radioactive chymotryptic peptide, Me-14C-labelled epsilon -N-mono-methyl-lysine, epsilon-N-dimethyl-lysine and epsilon-N-trimethyl-lysine were identified. The enzyme can easily be extracted from the N. crassa mycelial pads and was purified approx. 30-fold.