Modified electrode approaches for nitric oxide sensing
Modified electrode approaches for nitric oxide sensing
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DOI：
10.1016/s0039-9140(03)00360-6
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发表时间：
2003-09-30
期刊：
影响因子：
6.1
通讯作者：
Lorenzo, E
中科院分区：
文献类型：
--
作者：
Casero, E;Losada, J;Lorenzo, E
介绍了三种测定溶液中一氧化氮（NO）的方法。这些是基于，分别使用辣根过氧化物酶（HRP）生物传感器或氧化还原活性的过渡金属络合物的薄膜修饰的电极。在生物传感器的情况下，酶电化学固定到玻碳（GC）电极上。HRP的活性在NO的存在下被抑制。因此，活性的降低与溶液中存在的NO浓度相关。该生物传感器在2.7 × 10（-6）-1.1 × 10（-5）M NO范围内线性响应，检测限（5%抑制）为2.0 × 10（-6）M。在化学修饰电极的情况下，特别强调的是放在能够催化NO的氧化的材料。在电催化剂方面，讨论将集中在6，17-diferrocenyldibenzo[B，i] 5，9，14，18-tetra-aza[14]annulen]-镍（II）和铟（III）hexacyanoferrate（III）的电沉积膜。该传感器对NO的氧化表现出强而持久的电催化活性，检测限低（1 μ M），催化电流与NO浓度呈良好的线性关系.此外，由于硝酸盐和亚硝酸盐的存在而产生的干扰也显著减少。根据这些结果，所描述的修饰电极已被用作传感器，用于测定由非典型NO供体，如S-亚硝基-N-乙酰基-D，L-青霉胺（SNAP）分解产生的NO。所采用的各种方法的关键比较。(C)2003 Elsevier B. V.保留所有权利。
Three different methods for the determination of nitric oxide (NO) in solution are described. These are based, respectively, on the use of a horseradish peroxidase (HRP) biosensor or on electrodes modified with films of redox-active transition metal complexes. In the case of the biosensor the enzyme was electrochemically immobilized onto a glassy carbon (GC) electrode. The activity of HRP is inhibited in presence of NO. Thus, the decrease in activity is correlated to the concentration of NO present in solution. The biosensor responds linearly over the range of 2.7 x 10(-6)-1.1 x 10(-5) M NO with a detection limit (5% inhibition) of 2.0 x 10(-6) M. In the case of chemically modified electrodes, particular emphasis is placed on materials capable of catalyzing the oxidation of NO. In terms of electrocatalyst, the discussion will centre on electrodeposited films of 6,17-diferrocenyldibenzo[b,i]5,9,14,18-tetra-aza[14]annulen]-nickel(II) and indium(III) hexacyanoferrate(III). The resulting sensors exhibited potent and persistent electroacatalytic activity towards the oxidation of NO with low detection limits (1 muM) and good linear relationship between the catalytic current and NO concentrations. In addition, interference due to the presence of nitrate and nitrite have been significantly reduced. According to these results, the described modified electrodes have been used as sensors for the determination of NO generated by decomposition of atypical NO-donor, such as S-nitroso-N-acetyl-D,L-penicillamine (SNAP). A critical comparison of the various methodologies employed is made. (C) 2003 Elsevier B.V. All rights reserved.