Effect of 2-Bromostearate on Glucose-Phosphorylating Activities and the Dynamics of Insulin Secretion in Islets of Langerhans During Fasting
Effect of 2-Bromostearate on Glucose-Phosphorylating Activities and the Dynamics of Insulin Secretion in Islets of Langerhans During Fasting
复制标题
2-溴硬脂酸酯对禁食期间胰岛葡萄糖磷酸化活性和胰岛素分泌动态的影响
DOI：
10.2337/diab.33.9.858
复制
发表时间：
1984
期刊：
影响因子：
7.7
通讯作者：
R. Goberna
中科院分区：
文献类型：
--
作者：
F. Bedoya;R. Ramírez;E. Arilla;R. Goberna
Glucose-phosphorylating activity and insulin secretion were measured in homogenates of isolated rat islets and of perfused rat pancreas, respectively. Fasting for 96 h produced a significant decrease of both low-and high-Km glucose-phosphorylating activities and blocked the insulin secretory response to glucose. In the presence of glucose, 0.25 mM 2-bromostearate, a known inhibitor of fatty acid oxidation, partially restored the insulin response to glucose that was lost during fasting. This effect paralleled the restoration of glucose-phosphorylation activities (primarily the high–Km component) seen when islets isolated from 96-h-fasted rats were preincubated with 0.25 mM 2-bromostearate. It is concluded that fasting-induced adaptations of glucose-phosphorylating enzymes could account, at least in part, for the reduced insulin secretory response to glucose. 2-Bromostearate, an inhibitor of fatty acid oxidation, is able to restore both insulin secretory response and glucose-phosphorylating activities, suggesting possible interrelations among the correlated impairment in insulin secretion, islet glucose-phosphorylating activity, islet glucose metabolism, and the oxidation of fatty acids in the B-cell during fasting.
胰岛素、葡萄糖和环鸟苷酸在培养肝细胞葡萄糖激酶调节中的作用。
DOI：
--
发表时间：
1981
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
Spence,JT;Merrill,MJ;Pitot,HC
通讯作者：
Pitot,HC