Heparin structures in FGF-2-dependent morphological transformation of astrocytes.
Heparin structures in FGF-2-dependent morphological transformation of astrocytes.
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DOI：
10.1002/jbm.a.30338
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发表时间：
2005-09
期刊：
影响因子：
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通讯作者：
T. Nagayasu;S. Miyata;N. Hayashi;R. Takano;Y. Kariya;K. Kamei
中科院分区：
文献类型：
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作者：
T. Nagayasu;S. Miyata;N. Hayashi;R. Takano;Y. Kariya;K. Kamei
成纤维细胞生长因子 2 (FGF-2) 参与受伤大脑中神经胶质疤痕形成过程中星形胶质细胞（星状细胞）的形态转变。在本研究中，我们使用定量形态分析来研究肝素增强 FGF-2 诱导的培养皮质星形胶质细胞星状形成的结构要求。天然肝素显着促进FGF-2依赖性星形细胞星状形成，而肝素六糖抑制FGF-2依赖性星状细胞形成。此外，2-O-、6-O-和N-脱硫肝素不能促进FGF-2依赖性星状形成。由 FGF-2 或 FGF-2 与天然肝素的组合诱导的星状现象被 FGF 受体抑制剂 SU5402 抑制。这些结果表明，肝素的长度和硫酸化位置对于增强 FGF-2 依赖性星形胶质细胞星状形成很重要。此外，我们的研究结果表明，肝素寡糖可用于调节 FGF-2 依赖性星形胶质细胞转化。
Fibroblast growth factor-2 (FGF-2) participates in the morphological transformation of astrocytes (stellation) during the formation of glial scars in injured brains. In the current study, we used quantitative morphometric analysis to investigate the structural requirements for heparin's enhancement of FGF-2-induced stellation of cultured cortical astrocytes. Native heparin significantly promoted FGF-2-dependent astrocytic stellation, whereas heparin hexasaccharide inhibited FGF-2-dependent stellation. Furthermore, 2-O-, 6-O-, and N-desulfated heparins were unable to promote FGF-2-dependent stellation. The stellation induced by FGF-2 or by a combination of FGF-2 and native heparin was inhibited by SU5402, an FGF receptor inhibitor. These results demonstrate that the length and sulfated position of heparin are important for its enhancement of FGF-2-dependent astrocyte stellation. In addition, our findings show that heparin oligosaccharides are useful for regulating the FGF-2-dependent astrocytic transformation.