A gated relaxation oscillator mediated by FrzX controls morphogenetic movements in Myxococcus xanthus
A gated relaxation oscillator mediated by FrzX controls morphogenetic movements in Myxococcus xanthus
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DOI：
10.1038/s41564-018-0203-x
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发表时间：
2018-08-01
影响因子：
28.3
通讯作者：
Mignot, Tam
中科院分区：
文献类型：
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作者：
Guzzo, Mathilde;Murray, Sean M.;Mignot, Tam
细胞极性的动态控制对于细胞发育和运动的许多方面至关重要。在粘球菌xanthus，MgIA，G蛋白，和MgIB，其同源GTP酶激活蛋白，建立一个极性轴，定义了细胞的运动方向，并可以迅速逆转的Frz化学传感系统。尽管Frz对集体细胞行为至关重要，但Frz如何触发这一开关仍不清楚。在这里，我们使用遗传学，成像和数学建模表明，Frz通过门控弛豫振荡器控制极性反转。FrzX，我们确定为目标的Frz激酶，提供门控，从而作为触发逆转。极性蛋白RomR的缓慢重新定位然后产生不应期，在此期间另一个开关不能被触发。辅助Frz输出FrzZ可减少此延迟，允许在需要时快速反转。因此，这种架构导致高度可调谐的开关，其允许宽范围的反转频率。
Dynamic control of cell polarity is of critical importance for many aspects of cellular development and motility. In Myxococcus xanthus, MgIA, a G protein, and MgIB, its cognate GTPase-activating protein, establish a polarity axis that defines the direction of movement of the cell and that can be rapidly inverted by the Frz chemosensory system. Although vital for collective cell behaviours, how Frz triggers this switch has remained unknown. Here, we use genetics, imaging and mathematical modelling to show that Frz controls polarity reversals via a gated relaxation oscillator. FrzX, which we identify as a target of the Frz kinase, provides the gating and thus acts as the trigger for reversals. Slow relocalization of the polarity protein RomR then creates a refractory period during which another switch cannot be triggered. A secondary Frz output, FrzZ, decreases this delay, allowing rapid reversals when required. Thus, this architecture results in a highly tuneable switch that allows a wide range of reversal frequencies.