Bioluminescent imaging of Ca2+ activity reveals spatiotemporal dynamics in glial networks of dark-adapted mouse retina
Bioluminescent imaging of Ca2+ activity reveals spatiotemporal dynamics in glial networks of dark-adapted mouse retina
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DOI：
10.1113/jphysiol.2007.135715
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发表时间：
2007-09-15
影响因子：
5.5
通讯作者：
Brulet, Philippe
中科院分区：
文献类型：
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作者：
Agulhon, Cendra;Platel, Jean-Claude;Brulet, Philippe
胶质细胞 Ca2+ 兴奋性在神经元-胶质细胞的相互通讯中起着关键作用。在视网膜中，神经元-胶质细胞信号传导预计在黑暗中达到最大，但这种条件下的神经胶质 Ca2+ 信号特征尚未评估。为了解决这个问题，我们使用生物发光成像来选择性监测主要视网膜胶质细胞 Muller 细胞在黑暗条件下的自发 Ca2+ 变化。通过将这种成像方法与网络分析相结合，我们证明了 Muller 细胞的活动是在协同细胞网络中组织的，涉及位于远处和/或成簇的 2-16 个细胞。我们还报告说，小型网络（2-6 个穆勒细胞）的自发活动随时间重复，有时以相同的顺序排列，揭示了特定的时间动态。此外，我们还发现，TTX 抑制了共活性神经胶质细胞网络，表明神经节和/或无长突神经元细胞可能调节 Muller 细胞网络特性。这些结果首次证明，成年穆勒细胞的自发活动被模式化为相关网络，随着时间的推移，这些网络显示出重复的共激活序列。此外，我们的生物发光技术提供了一种新的工具来研究黑暗条件下视网膜中胶质Ca2+事件的动态特征，这将极大地促进视网膜暗适应过程的未来研究。
Glial Ca2+ excitability plays a key role in reciprocal neuron-glia communication. In the retina, neuron-glia signalling is expected to be maximal in the dark, but the glial Ca2+ signal characteristics under such conditions have not been evaluated. To address this question, we used bioluminescence imaging to monitor spontaneous Ca2+ changes under dark conditions selectively in Muller cells, the principal retinal glial cells. By combining this imaging approach with network analysis, we demonstrate that activity in Muller cells is organized in networks of coactive cells, involving 2-16 cells located distantly and/or in clusters. We also report that spontaneous activity of small networks (2-6 Muller cells) repeat over time, sometimes in the same sequential order, revealing specific temporal dynamics. In addition, we show that networks of coactive glial cells are inhibited by TTX, indicating that ganglion and/or amacrine neuronal cells probably regulate Muller cell network properties. These results represent the first demonstration that spontaneous activity in adult Muller cells is patterned into correlated networks that display repeated sequences of coactivations over time. Furthermore, our bioluminescence technique provides a novel tool to study the dynamic characteristics of glial Ca2+ events in the retina under dark conditions, which should greatly facilitate future investigations of retinal dark-adaptive processes.