Ligand-recognizing motifs in plant LysM receptors are major determinants of specificity
Ligand-recognizing motifs in plant LysM receptors are major determinants of specificity
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DOI：
10.1126/science.abb3377
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发表时间：
2020-08-07
期刊：
影响因子：
56.9
通讯作者：
Radutoiu, Simona
中科院分区：
文献类型：
--
作者：
Bozsoki, Zoltan;Gysel, Kira;Radutoiu, Simona
植物进化赖氨酸基序（LysM）受体识别和解析微生物激发子，并驱动细胞内信号传导，以限制或促进微生物定植。我们研究了百脉根几丁质和结瘤因子受体如何启动免疫或根瘤共生的差异信号。这些受体的LysM1结构域中的两个基序决定配体的特异性识别并区分它们在植物中的功能。这些基序定义配体结合位点，并构成同源结瘤因子受体中结构上最不同的区域。一个相邻的基序调节结瘤因子识别的特异性，并决定了在豆科植物中选择兼容的根瘤菌共生体。我们还确定了LysM受体的结合特异性如何改变，以促进结瘤因子的识别和信号从几丁质受体，推进工程根瘤菌共生到非豆科植物的前景。
Plants evolved lysine motif (LysM) receptors to recognize and parse microbial elicitors and drive intracellular signaling to limit or facilitate microbial colonization. We investigated how chitin and nodulation (Nod) factor receptors of Lotus japonicas initiate differential signaling of immunity or root nodule symbiosis. Two motifs in the LysM1 domains of these receptors determine specific recognition of ligands and discriminate between their in planta functions. These motifs define the ligand-binding site and make up the most structurally divergent regions in cognate Nod factor receptors. An adjacent motif modulates the specificity for Nod factor recognition and determines the selection of compatible rhizobial symbionts in legumes. We also identified how binding specificities in LysM receptors can be altered to facilitate Nod factor recognition and signaling from a chitin receptor, advancing the prospects of engineering rhizobial symbiosis into nonlegumes.