Evidence for Rapid Oxidative Phosphorylation and Lactate Fermentation in Motile Human Sperm by Hyperpolarized (13)C Magnetic Resonance Spectroscopy.
Evidence for Rapid Oxidative Phosphorylation and Lactate Fermentation in Motile Human Sperm by Hyperpolarized (13)C Magnetic Resonance Spectroscopy.
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DOI：
10.1038/s41598-017-04146-1
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发表时间：
2017-06-28
影响因子：
4.6
通讯作者：
Paley MNJ
中科院分区：
文献类型：
--
作者：
Reynolds S;Ismail NFB;Calvert SJ;Pacey AA;Paley MNJ
精子活力差是男性不育的常见原因，对此没有经验性治疗。精子活力是由腺苷三磷酸，但乳酸发酵和氧化磷酸化（OxPhos）的相对重要性是有争议的。为了研究能量代谢与精子活力之间的关系，我们首次使用溶解动态核极化（dDNP）来显示13 C1-丙酮酸盐快速转化为乳酸盐和碳酸氢盐，表明精子中的活性糖酵解和OxPhos代谢。乳酸和碳酸氢盐信号的大小与进行性运动精子的浓度呈正相关。在控制精子浓度后，增加的前向精子活力产生更多的丙酮酸转化为乳酸盐和碳酸氢盐。dDNP技术允许在精子生命的不同阶段跟踪精子代谢的“快照”。这可能有助于揭示精子活力差的原因，并为新的治疗或疗法提出新的方法。
Poor sperm motility is a common cause of male infertility for which there are no empirical therapies. Sperm motility is powered by adenosine triphosphate but the relative importance of lactate fermentation and Oxidative Phosphorylation (OxPhos) is debated. To study the relationship between energy metabolism and sperm motility we used dissolution Dynamic Nuclear Polarization (dDNP) for the first time to show the rapid conversion of 13C1-pyruvate to lactate and bicarbonate, indicating active glycolytic and OxPhos metabolism in sperm. The magnitude of both lactate and bicarbonate signals were positively correlated with the concentration of progressively motile sperm. After controlling for sperm concentration, increased progressive sperm motility generated more pyruvate conversion to lactate and bicarbonate. The technique of dDNP allows ‘snapshots’ of sperm metabolism to be tracked over the different stages of their life. This may provide help to uncover the causes of poor sperm motility and suggest new approaches for novel treatments or therapies.