Alterations of histone modifications at the senescence-associated gene HvS40 in barley during senescence
Alterations of histone modifications at the senescence-associated gene HvS40 in barley during senescence
复制标题
DOI：
10.1007/s11103-015-0358-2
复制
发表时间：
2015-09-01
影响因子：
5.1
通讯作者：
Humbeck, Klaus
中科院分区：
文献类型：
--
作者：
Ay, Nicole;Janack, Bianka;Humbeck, Klaus
大麦基因HvS40编码一个推定的叶片衰老调节因子，在叶片衰老过程中被强烈诱导。如染色质免疫沉淀所示，衰老开始后，常染色质组蛋白修饰H3K9ac被添加在靠近ATG的启动子和HvS40的编码序列处。同时，异染色质H3K9me2的水平在该基因处降低。亚硫酸氢盐测序显示，在这个区域没有DNA甲基化，但高度甲基化的DNA岛，从翻译起始位点上游664 bp开始，成熟和衰老的叶片。衰老叶片中DNA甲基化的减少可以在该甲基化岛末端的一个特定CpG基序处显示。此外，通过免疫细胞学分析了衰老过程中染色质结构的整体变化，揭示了细胞核中异染色质H3K9me2模式空间分布的衰老相关变化。我们的研究结果证明了一个衰老特异性机制，改变组蛋白修饰标记在不同序列的衰老相关基因HvS40和改变分布的异染色质区的细胞核。
The barley gene HvS40, encoding a putative regulator of leaf senescence, is strongly induced during leaf senescence. As shown by chromatin immunoprecipitation, euchromatic histone modification H3K9ac is added at promoter close to ATG and coding sequence of HvS40 after onset of senescence. In parallel, level of heterochromatic H3K9me2 decreases at this gene. Bisulfite sequencing revealed no DNA-methylation in this region, but a heavily methylated DNA-island, starting 664 bp upstream from translational start site in both, mature and senescent leaves. A decrease in DNA methylation in senescing leaves could be shown at one specific CpG motif at the end of this methylation island. In addition, global changes in chromatin structure during senescence were analyzed via immunocytology, revealing senescence-associated changes in spatial distribution of heterochromatic H3K9me2 patterns in the nuclei. Our results prove a senescence-specific mechanism, altering histone modification marks at distinct sequences of the senescence-associated gene HvS40 and altering distribution of heterochromatic areas in the nuclei.