Recovery of nitrification and production of NO and N2O after exposure of soil to acetylene
Recovery of nitrification and production of NO and N2O after exposure of soil to acetylene
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DOI：
10.1007/s003740050277
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发表时间：
1997-05
影响因子：
6.5
通讯作者：
A. Bollmann;R. Conrad
中科院分区：
文献类型：
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作者：
A. Bollmann;R. Conrad
在低乙炔分压（10 Pa）下培养土壤是一种广泛使用的特异性抑制硝化作用的方法，这是由于硝化细菌对铵单加氧酶（AMO）的自杀性抑制，AMO是硝化细菌氧化NH 4+的第一个酶。虽然AMO的抑制是不可逆的，但如果合成新的酶，则有可能恢复活性。在三种不同土壤的实验中，从大气中去除乙炔后不久，NH 4+浓度下降，NO3-浓度上升。在除去乙炔后立即开始恢复NO的生产。仅用10 Pa乙炔预培养的土壤样品中NO和N2 O的释放速率高于在10 Pa乙炔存在下保持的土壤样品。在10 Pa乙炔的永久存在下，NH 4+和NO3-浓度保持恒定，NO和N2 O的释放速率较低。这些低释放率显然是由于硝化以外的过程。我们的实验表明，低（10帕）乙炔分压的硝化作用的封锁是唯一可靠的，当土壤保持永久接触乙炔。
Incubation of soil under low partial pressures of acetylene (10 Pa) is a widely used method to specifically inhibit nitrification due to the suicide inhibition of ammonium monooxygenase (AMO), the first enzyme in NH4+oxidation by nitrifying bacteria. Although the inhibition of AMO is irreversible, recovery of activity is possible if new enzyme is synthesized. In experiments with three different soils, NH4+concentrations decreased and NO3–concentrations increased soon after acetylene was removed from the atmosphere. Recovery of NO production started immediately after the removal of acetylene. The release rates of NO and N2O were higher in soil samples which were only preincubated with 10 Pa acetylene than in those which were kept in the presence of 10 Pa acetylene. In the permanent presence of 10 Pa acetylene, NH4+and NO3–concentrations stayed constant, and the release rates of NO and N2O were low. These low release rates were apparently due to processes other than nitrification. Our experiments showed that the blockage of nitrification by low (10 Pa) acetylene partial pressures is only reliable when the soil is kept in permanent contact with acetylene.