Guanabenz inhibits alveolar bone resorption in a rat model of periodontitis
Guanabenz inhibits alveolar bone resorption in a rat model of periodontitis
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DOI：
10.1016/j.jphs.2021.08.003
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发表时间：
2021-08-26
影响因子：
3.5
通讯作者：
Goto, Shigemi
中科院分区：
文献类型：
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作者：
Muramatsu, Ryujiro;Sato, Takuma;Goto, Shigemi
已知交感神经活性的增加通过促进骨吸收而加重骨质疏松症。然而，它在很大程度上是未知的交感神经活动参与牙周炎的恶化。在这项研究中，我们研究了α（2）-肾上腺素能受体（α（2）-AR）激动剂胍那苄（guanabenz）是否能降低交感神经活性，减弱牙周炎大鼠高交感神经活性的牙槽骨吸收。分别用显微CT和苏木精-伊红染色法观察牙周组织中残留牙槽骨的形态和附着水平。此外，使用抗酒石酸酸性磷酸酶染色测量每个骨表面的破骨细胞数量和每个骨表面的破骨细胞表面。为了检测胍那苄对促炎细胞因子的抑制作用，使用MTT染色测量牙周组织中酪氨酸羟化酶（TH）、TNF-α、IL-1 β和IL-6的表达水平。胍那苄的管理衰减的损失牙槽骨和附着水平的大鼠具有高交感神经活性。此外，它的管理抑制破骨细胞的数量在大鼠具有高交感神经活性。用胍那苄处理12周的大鼠牙周组织中TH、TNF-α、IL-1 β和IL-6阳性细胞低于具有高交感神经活性的对照大鼠。本研究表明，α（2）-AR激动剂胍那苄通过降低大鼠交感神经活性来减弱牙槽骨吸收。(C)2021年，任作家。Elsevier B. V.代表日本药理学会制作和主办。
Increase of sympathetic activity has been known to exacerbate osteoporosis through promotion of bone resorption. However, it is largely unknown about involvement of sympathetic activity in exacerbation of periodontitis. In this study, we investigated whether alpha(2)-adrenergic receptor (alpha(2)-AR) agonist guanabenz which decreases sympathetic activity, attenuates alveolar bone resorption in rats having high sympathetic activity with periodontitis. Volumes of residual alveolar bone and attachment levels in periodontium were examined using micro-computed tomography and hematoxylin-eosin staining, respectively. Furthermore, osteoclast numbers per bone surface and osteoclast surface per bone surface were measured using tartrate-resistant acid phosphatase staining. To examine the suppressive effects of guanabenz on pro-inflammatory cytokines, expression levels of tyrosine hydroxylase (TH), TNF-alpha, IL-1 beta, and IL-6 in periodontium were measured using immunohistostaining. Administration of guanabenz attenuated loss of alveolar bone and attachment levels in rats having high sympathetic activity. Furthermore, its administration suppressed osteoclast numbers in rats having high sympathetic activity. TH, TNF-alpha, IL-1 beta, and IL-6 positive cells in periodontium in rats treated with guanabenz for 12 weeks, were lower than those in control rats having high sympathetic activity. This study demonstrated administration of alpha(2)-AR agonist guanabenz attenuates alveolar bone resorption through decrease of sympathetic activity in rats. (C) 2021 The Authors. Production and hosting by Elsevier B.V. on behalf of Japanese Pharmacological Society.