Trace analysis of multi-class pesticide residues in Chinese medicinal health wines using gas chromatography with electron capture detection.
Trace analysis of multi-class pesticide residues in Chinese medicinal health wines using gas chromatography with electron capture detection.
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DOI：
10.1038/srep21558
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发表时间：
2016-02-17
影响因子：
4.6
通讯作者：
Yang MH
中科院分区：
文献类型：
--
作者：
Kong WJ;Liu QT;Kong DD;Liu QZ;Ma XP;Yang MH
描述了一种使用基于 QuEChERS（快速、简单、廉价、有效、耐用和安全）的萃取方法和气相色谱-电子捕获检测 (GC-ECD) 对中药 (CM) 保健酒中 22 种有机氯农药 (OCP) 和 5 种拟除虫菊酯农药 (PYP) 进行多残留、高通量测定的方法。优化了多个参数以缩短制备和分离时间，同时仍保持高灵敏度。加标样品的验证测试表明，27 种农药在较宽的浓度范围内具有良好的线性 (R = 0.9909–0.9996)。检测限 (LOD) 和定量限 (LOQ) 在 ng/L 水平上测量，OCP 分别为 0.06–2ng/L 和 0.2–6ng/L，PYP 分别为 0.02–3ng/L 和 0.06–7ng/L。日间和日内精密度测试显示，OCP 的变化分别为 0.65-9.89%，PYP 的变化为 0.98-13.99%。平均回收率在 47.74-120.31% 范围内，相对标准偏差低于 20%。然后将开发的方法应用于分析 80 CM 的葡萄酒样品。 Beta-BHC（六氯化苯）是最常检测到的农药，浓度水平为 5.67–31.55mg/L，其次是 delta-BHC、反式氯丹、γ-BHC 和 α-BHC。经验证的方法简单且经济，对痕量多类农药具有足够的灵敏度。实验室可以采用它来进行这种和其他类型的复杂基质分析。
A method is described for multi-residue, high-throughput determination of trace levels of 22 organochlorine pesticides (OCPs) and 5 pyrethroid pesticides (PYPs) in Chinese medicinal (CM) health wines using a QuEChERS (quick, easy, cheap, effective, rugged, and safe) based extraction method and gas chromatography-electron capture detection (GC-ECD). Several parameters were optimized to improve preparation and separation time while still maintaining high sensitivity. Validation tests of spiked samples showed good linearities for 27 pesticides (R = 0.9909–0.9996) over wide concentration ranges. Limits of detection (LODs) and quantification (LOQs) were measured at ng/L levels, 0.06–2 ng/L and 0.2–6 ng/L for OCPs and 0.02–3 ng/L and 0.06–7 ng/L for PYPs, respectively. Inter- and intra-day precision tests showed variations of 0.65–9.89% for OCPs and 0.98–13.99% for PYPs, respectively. Average recoveries were in the range of 47.74–120.31%, with relative standard deviations below 20%. The developed method was then applied to analyze 80 CM wine samples. Beta-BHC (Benzene hexachloride) was the most frequently detected pesticide at concentration levels of 5.67–31.55 mg/L, followed by delta-BHC, trans-chlordane, gamma-BHC, and alpha-BHC. The validated method is simple and economical, with adequate sensitivity for trace levels of multi-class pesticides. It could be adopted by laboratories for this and other types of complex matrices analysis.