A global view of gene activity and alternative splicing by deep sequencing of the human transcriptome
A global view of gene activity and alternative splicing by deep sequencing of the human transcriptome
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DOI：
10.1126/science.1160342
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发表时间：
2008-08-15
期刊：
影响因子：
56.9
通讯作者：
Yaspo, Marie-Laure
中科院分区：
文献类型：
--
作者：
Sultan, Marc;Schulz, Marcel H.;Yaspo, Marie-Laure
人类转录组的功能复杂性尚未完全阐明。我们报告了人类胚胎肾脏和B细胞系的人类转录组的高通量序列。我们使用转录本的shot弹枪测序来生成随机分布的读取。其中，有50％映射到独特的基因组位置，其中80％对应于已知的外显子。我们发现，映射到已知基因的聚腺苷酸化转录组的66％，未经宣传的基因组区域的34％。根据已知的转录本，RNA-SEQ比微阵列可以检测到25％的基因。对信使RNA剪接事件的全球调查确定了94,241个剪接连接（以前4096个以前未识别），并表明外显子跳过是最普遍的替代剪接形式。
The functional complexity of the human transcriptome is not yet fully elucidated. We report a high- throughput sequence of the human transcriptome from a human embryonic kidney and a B cell line. We used shotgun sequencing of transcripts to generate randomly distributed reads. Of these, 50% mapped to unique genomic locations, of which 80% corresponded to known exons. We found that 66% of the polyadenylated transcriptome mapped to known genes and 34% to nonannotated genomic regions. On the basis of known transcripts, RNA- Seq can detect 25% more genes than can microarrays. A global survey of messenger RNA splicing events identified 94,241 splice junctions ( 4096 of which were previously unidentified) and showed that exon skipping is the most prevalent form of alternative splicing.