IDENTIFICATION OF A STAPHYLOCOCCUS-AUREUS TRANSPOSON (TN4291) THAT CARRIES THE METHICILLIN RESISTANCE GENE(S)
IDENTIFICATION OF A STAPHYLOCOCCUS-AUREUS TRANSPOSON (TN4291) THAT CARRIES THE METHICILLIN RESISTANCE GENE(S)
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DOI：
10.1128/jb.170.1.149-154.1988
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发表时间：
1988-01-01
影响因子：
3.2
通讯作者：
IANDOLO, JJ
中科院分区：
文献类型：
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作者：
TREES, DL;IANDOLO, JJ
我们在铅笔酶质粒pI524的二级插入位点分离到一个携带甲氧西林耐药基因的转座子（Tn4291）。在四环素耐药质粒pSN1从甲氧西林耐药供体转导到染色体初级位点携带mec等位基因的受体时，Tn4291转位到pI524。Tn4291的插入引起pI524的广泛重排，并导致形成一个27.9千碱基对的质粒（pIT103），该质粒编码对甲氧西林和镉的抗性，但不编码对青霉素的抗性。虽然对甲氧西林和镉的抗性总是联系在一起，但Tn4291只有在染色体mec等位基因存在的情况下才能稳定维持，而在没有mec等位基因的情况下，质粒是不稳定的，并且会转位到原发位点。随后，形成了一个20.1千碱基对的质粒pIT203，该质粒保留了抗镉性，并恢复了表达。β的能力。内酰胺酶的活动。
We isolated a transposon (Tn4291) that carried the resistance gene(s) for methicillin in a secondary insertion site on the pencillinase plasmid pI524. Transposition of Tn4291 into pI524 occurred during the transduction of the tetracycline resistance plasmid pSN1 from a methicillin-resistant donor into a recipient that carried the mec allele in the primary site on the chromosome. Insertion of Tn4291 caused extensive rearrangement of pI524 and resulted in the formation of a 27.9-kilobase-pair plasmid (pIT103) which coded for resistance to methicillin and cadmium, but not penicillin. Although resistance to methicillin and cadmium were always linked, Tn4291 was stably maintained only in the presence of a chromosomal mec allele, while in its absence the plasmid was unstable and transposition to the primary site occurred. Subsequently, a 20.1-kilobase-pair plasmid, pIT203, was formed which retained cadmium resistance and regained the ability to express .beta.-lactamase activity.