Biodiversity of N-cycle bacteria in nitrogen removing moving bed biofilters for freshwater recirculating aquaculture systems.
Biodiversity of N-cycle bacteria in nitrogen removing moving bed biofilters for freshwater recirculating aquaculture systems.
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DOI：
10.1016/j.aquaculture.2010.05.019
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发表时间：
2010-08
期刊：
影响因子：
4.5
通讯作者：
M. V. Kessel;H. Harhangi;K. Pas-Schoonen;J. Vossenberg;G. Flik;M. Jetten;P. Klaren;H. J. M. Camp
中科院分区：
文献类型：
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作者：
M. V. Kessel;H. Harhangi;K. Pas-Schoonen;J. Vossenberg;G. Flik;M. Jetten;P. Klaren;H. J. M. Camp
维持最佳水质和去除氮化合物对全球水产养殖业构成挑战。对与锦鲤池连接的移动床生物膜反应器（MBBR）生物膜中参与氮循环的不同细菌的存在和活性进行了实验研究。对于该MBBR系统，在活性测定中发现每天的氮去除率为3.5g，而计算出的预测氮去除率为每天58g。通过原位杂交（FISH）分析证实了生物膜中存在氨氧化菌和亚硝酸盐氧化菌。在反应器的生物膜中发现了大量的植物菌，但它们不能与已知厌氧氨氧化菌属的寡核苷酸探针杂交。对MBBR生物膜的分子16S rRNA基因分析显示，该生物过滤器中存在一组新的植物菌细胞，这可能解释了实验发现和计算的系统氮去除率的差异。新的16S rRNA基因序列与所描述的厌氧氨氧化菌具有较低的相似性。这项研究和其他研究可能意味着厌氧氨氧化反应可以纳入水产养殖淡水系统的生物过滤系统，这使得以相对廉价和可持续的方式从这些系统中去除氮化合物成为可能。
Maintenance of optimal water quality and removal of nitrogen compounds pose challenges to aquaculture worldwide. Presence and activity of different bacteria involved in nitrogen cycling in the biofilm of a moving bed biofilm reactor (MBBR) connected to a Koi carp tank were investigated experimentally. For this MBBR system, a nitrogen removing rate of 3.5g nitrogen per day was found in activity assays, whereas the predicted nitrogen removal rate was calculated to be 58g nitrogen per day. The presence of ammonium-oxidizing and nitrite-oxidizing bacteria in the biofilm was demonstrated by situ hybridization (FISH) analysis. A large number of planctomycetes were shown to be present in the biofilm of the reactor, but they did not hybridize to oligonucleotide probes of known genera of anammox bacteria. Molecular 16S rRNA gene analysis of the MBBR biofilm revealed a novel group of planctomycete cells was present in this biofilter, which may explain the difference in experimentally found and calculated nitrogen removal of the system. The novel 16S rRNA gene sequences showed a low similarity to described anammox bacteria. This and other studies may implicate that the anammox reaction can be incorporated in biofilter systems for aquacultural freshwater systems, which makes it possible to remove nitrogen compounds from these systems in a relatively inexpensive and sustainable manner.