Melatonin inhibits embryonic salivary gland branching morphogenesis by regulating both epithelial cell adhesion and morphology.
Melatonin inhibits embryonic salivary gland branching morphogenesis by regulating both epithelial cell adhesion and morphology.
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DOI：
10.1371/journal.pone.0119960
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发表时间：
2015
期刊：
影响因子：
3.7
通讯作者：
Sakai T
中科院分区：
文献类型：
--
作者：
Obana-Koshino A;Ono H;Miura J;Sakai M;Uchida H;Nakamura W;Nohara K;Maruyama Y;Hattori A;Sakai T
许多器官，包括唾液腺、肺和肾，在胚胎发育期间由上皮分支形成。分支形态发生通过局部生长或形成将上皮细胞细分为芽的裂缝而发生。这一过程是由各种因素促进的，但分枝形态建成的机制还不完全清楚。在这里，我们已经定义褪黑激素作为一个潜在的负调节器或分支形态发生的“刹车”，表明它和它的受体水平下降时，分支形态开始，并确定了它调节的过程。褪黑激素具有多种生理功能，包括昼夜节律调节、自由基清除和性腺发育。此外，褪黑激素存在于唾液中，并且可能在口腔中具有重要的生理作用。在本研究中，我们发现褪黑激素受体是高度表达的腺泡上皮细胞的胚胎下颌下腺。我们还发现，外源性褪黑激素减少唾液腺的大小和抑制分支形态发生。我们认为，这种抑制不依赖于增殖或凋亡的变化，而是与上皮细胞粘附和形态的变化。总之，我们已经证明了褪黑激素在胚胎发育过程中的器官形成的新功能。
Many organs, including salivary glands, lung, and kidney, are formed by epithelial branching during embryonic development. Branching morphogenesis occurs via either local outgrowths or the formation of clefts that subdivide epithelia into buds. This process is promoted by various factors, but the mechanism of branching morphogenesis is not fully understood. Here we have defined melatonin as a potential negative regulator or “brake” of branching morphogenesis, shown that the levels of it and its receptors decline when branching morphogenesis begins, and identified the process that it regulates. Melatonin has various physiological functions, including circadian rhythm regulation, free-radical scavenging, and gonadal development. Furthermore, melatonin is present in saliva and may have an important physiological role in the oral cavity. In this study, we found that the melatonin receptor is highly expressed on the acinar epithelium of the embryonic submandibular gland. We also found that exogenous melatonin reduces salivary gland size and inhibits branching morphogenesis. We suggest that this inhibition does not depend on changes in either proliferation or apoptosis, but rather relates to changes in epithelial cell adhesion and morphology. In summary, we have demonstrated a novel function of melatonin in organ formation during embryonic development.
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影响因子：
10.3
作者：
Martínez-Campa, C;Alonso-González, C;Sánchez-Barceló, EJ
通讯作者：
Sánchez-Barceló, EJ
DOI：
10.1126/science.1191880
发表时间：
2010-07-30
期刊：
Science (New York, N.Y.)
影响因子：
--
作者：
Onodera T;Sakai T;Hsu JC;Matsumoto K;Chiorini JA;Yamada KM
通讯作者：
Yamada KM
影响因子：
10.3
作者：
Jung-Hynes B;Schmit TL;Reagan-Shaw SR;Siddiqui IA;Mukhtar H;Ahmad N
通讯作者：
Ahmad N
影响因子：
14
作者：
Miyajima, Hiroyuki;Matsumoto, Takuya;Imazato, Satoshi
通讯作者：
Imazato, Satoshi
影响因子：
6.1
作者：
Naji, L;Canillo-Vico, A;Calvo, JR
通讯作者：
Calvo, JR