An Optimized Whole‐Mount Immunofluorescence Method for Shoot Apices
An Optimized Whole‐Mount Immunofluorescence Method for Shoot Apices
复制标题
一种优化的芽尖整体免疫荧光法
DOI：
10.1002/cpz1.101
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发表时间：
2021
期刊：
影响因子：
--
通讯作者：
Jackson, David
中科院分区：
文献类型：
--
作者：
Tran, Thu M.;Demesa‐Arevalo, Edgar;Kitagawa, Munenori;Garcia‐Aguilar, Marcelina;Grimanelli, Daniel;Jackson, David
蛋白质的定位提供了关于其生物学功能的重要信息。通过免疫荧光技术对蛋白质进行可视化已经成为细胞生物学中的一种重要方法。在这里，我们描述了一个易于遵循的免疫荧光协议，定位蛋白质在整个安装组织的玉米（玉米）和拟南芥。我们以玉米穗原基和拟南芥花序顶端为例，介绍了整体贴装免疫荧光程序，然后是每个步骤的提示和建议。此外，我们提供了一个支持协议来描述使用ImageJ插件来分析共定位。该方案已被优化以观察2 - 5 mm玉米穗原基或发育中的拟南芥花序顶端中的蛋白质;然而，它可以用作在其他植物组织和物种中进行整体免疫荧光的参考。© 2021 Wiley Periodicals LLC.基本方案：玉米和拟南芥茎尖的整体免疫荧光支持方案：通过ImageJ中的JACoP插件测量共定位
The localization of a protein provides important information about its biological functions. The visualization of proteins by immunofluorescence has become an essential approach in cell biology. Here, we describe an easy‐to‐follow immunofluorescence protocol to localize proteins in whole‐mount tissues of maize (Zea mays) and Arabidopsis. We present the whole‐mount immunofluorescence procedure using maize ear primordia and Arabidopsis inflorescence apices as examples, followed by tips and suggestions for each step. In addition, we provide a supporting protocol to describe the use of an ImageJ plug‐in to analyze colocalization. This protocol has been optimized to observe proteins in 2‐5 mm maize ear primordia or in developing Arabidopsis inflorescence apices; however, it can be used as a reference to perform whole‐mount immunofluorescence in other plant tissues and species. © 2021 Wiley Periodicals LLC.Basic Protocol: Whole‐mount immunofluorescence for maize and Arabidopsis shoot apicesSupport Protocol: Measure colocalization by JACoP plugin in ImageJ
影响因子：
48
作者：
Schindelin, Johannes;Arganda-Carreras, Ignacio;Frise, Erwin;Kaynig, Verena;Longair, Mark;Pietzsch, Tobias;Preibisch, Stephan;Rueden, Curtis;Saalfeld, Stephan;Schmid, Benjamin;Tinevez, Jean-Yves;White, Daniel James;Hartenstein, Volker;Eliceiri, Kevin;Tomancak, Pavel;Cardona, Albert
通讯作者：
Cardona, Albert
DOI：
10.1007/978-1-62703-221-6_14
发表时间：
2013-01-01
期刊：
Methods in molecular biology (Clifton, N.J.)
影响因子：
--
作者：
Forestan, Cristian;Carraro, Nicola;Varotto, Serena
通讯作者：
Varotto, Serena