THE POLYPEPTIDE STRUCTURE OF THE SM AND RNP NUCLEAR ANTIGENS
THE POLYPEPTIDE STRUCTURE OF THE SM AND RNP NUCLEAR ANTIGENS
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DOI：
10.1016/0161-5890(83)90130-x
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发表时间：
1983-01-01
影响因子：
3.6
通讯作者：
COHEN, PL
中科院分区：
文献类型：
--
作者：
EISENBERG, RA;KLAPPER, DG;COHEN, PL
Sm and nuclear ribonucleoprotein [RNP] are ubiquitous nuclear antigens towards which important autoantibodies are directed in systemic lupus erythematosus and other human autoimmune syndromes. Physicochemical techniques and affinity adsorptions were used to purify the polypeptide components of these antigens. The Sm antigen contained polypeptide chains of 15,000 and 17,000 MW. The RNP antigen, which is known by immunochemical techniques to contain the Sm antigen, had the same 2 polypeptides and a larger one of 85,000 MW. This larger peptide was quite labile and apparently broke down into smaller components with manipulation. The process of affinity purification of the Sm polypeptides gave a product which had increased positive charge. Amino acid analysis of the Sm polypeptides confirmed the presence of relatively large numbers of basic residues. The purified Sm antigen provided an effective reagent for the investigation of autoreactivity to Sm. The differences in structure from the results and others published are probably accounted for by the lability of the constituent polypeptides.
