HPLC Determination of the Antiseptic Agent Chlorhexidine and its Degradation Products 4-Chloroaniline and 1-Chloro-4-Nitrobenzene in Serum and Urine
HPLC Determination of the Antiseptic Agent Chlorhexidine and its Degradation Products 4-Chloroaniline and 1-Chloro-4-Nitrobenzene in Serum and Urine
复制标题
高效液相色谱法测定血清和尿液中抗菌剂洗必太及其降解产物4-氯苯胺和1-氯-4-硝基苯
DOI：
10.1007/s00604-004-0194-6
复制
发表时间：
2004
影响因子：
5.7
通讯作者：
A. Kramer
中科院分区：
文献类型：
--
作者：
H. Below;N. Lehan;A. Kramer
氯己定（Chlorhexidine，1，1 ′-hexamethylenebis-[5-（p-chlorophenyl）-biguanide]-digluconate; CHD）是常用的防腐剂之一。尽管如此，其吸收和消除几乎没有研究。其中一个主要原因是缺乏合适的程序来分析生物材料。CHD样品中也可能含有致癌物4-氯苯胺，4-氯苯胺也可能是CHD的降解产物，本文建立了CHD、4-氯苯胺和1-氯-4-硝基苯（4-氯苯胺的降解产物）的高效液相色谱分离方法。还测试了用于分离血清和尿液中分析物的固相萃取和脱蛋白方法。在后一种方法中，使用单一程序从生物材料中分离所有三种化合物是不成功的。分离冠心病，另一方面，无论是从血清和尿液中使用弱阳离子交换剂的固相萃取是成功的，并表现出可靠的检索率。
Chlorhexidine (1,1′-hexamethylene-bis-[5-(p-chlorophenyl)-biguanide]-digluconate; CHD) is one of the most frequently used antiseptics. In spite of this, its resorption and elimination has hardly been investigated. One of the main reasons for this is the lack of suitable procedures for its analysis in biological materials. Samples of CHD also may contain the carcinogen 4-chloroaniline which also may be formed as a degradation product in preparations.A HPLC method with end-capped stationary phase was developed which separates CHD, 4-chloroaniline and 1-chloro-4-nitrobenzene (a degradation product of 4-chloroaniline). Methods of solid-phase extraction and deproteinization for the purpose of isolating the analytes in serum and urine were also tested. In the latter process, isolation of all three compounds from the biological material using a single procedure was unsuccessful. Isolation of CHD, on the other hand, both from serum and from urine using solid-phase extraction with weak cation exchangers was successful and showed reliable retrieval rates.