DISPERSED DISCRETE LENGTH POLYMORPHISM OF MITOCHONDRIAL-DNA IN THE SCALLOP PLACOPECTEN-MAGELLANICUS (GMELIN)

DISPERSED DISCRETE LENGTH POLYMORPHISM OF MITOCHONDRIAL-DNA IN THE SCALLOP PLACOPECTEN-MAGELLANICUS (GMELIN)
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DOI:
10.1007/bf00310901
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发表时间:
1993-04-01
期刊:
影响因子:
2.5
通讯作者:
ZOUROS, E
ZOUROS, E
中科院分区:
生物学3区
文献类型:
--
作者:
FULLER, KM;ZOUROS, E

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在麦哲伦扇贝线粒体基因组中发现了三个不同的区域,表现出长度递增多态性。每个基因座具有离散且不同的变异单位,在基因座I处测量为1450 bp,在基因座II处为250 bp,并且在基因座III处小于100 bp。在每个位点至少观察到6个大小类别,个体变异可以解释种内mtDNA大小范围为31-42 kb,但不能解释基因组异常大的碱基大小。与两个可变区的克隆的分子内杂交模式表明,有一个分散的序列相似性与1,450 bp的位点-I重复和它的侧翼,并与位点II的一些部分。
Three separate regions exhibiting incremental length polymorphism have been found in the mitochondrial genome of the scallop Placopecten magellanicus. Each locus has a discrete and different unit of variation, measuring 1450 bp at locus I, 250 bp at locus II, and less than 100 bp at locus III. At least six size classes were observed at each locus, and individual variation can account for the intraspecific mtDNA size range of 31-42 kb, but not for the unusually large base size of the genome. Intramolecular hybridization patterns with clones of two of the variable regions indicate that there is a dispersed sequence similarity with the 1,450-bp locus-I repeat and its flanks, and with some part of locus II.