Tissue engineering based on electrochemical desorption of an RGD-containing oligopeptide
Tissue engineering based on electrochemical desorption of an RGD-containing oligopeptide
复制标题
DOI：
10.1002/term.519
复制
发表时间：
2013-03-01
影响因子：
3.3
通讯作者：
Fukuda, Junji
中科院分区：
文献类型：
--
作者：
Mochizuki, Naoto;Kakegawa, Takahiro;Fukuda, Junji
本文描述了一种通过特定寡肽有效分离粘附在金基质上的细胞的非侵入性方法。分离是通过电刺激实现的。寡肽含有半胱氨酸，它会在金表面自发形成硫醇金键。将镀金基质浸入含有肽的溶液后，这种化学吸附在 10 分钟内达到> 95% 平衡。施加负电位后 5 分钟内，肽可逆地从表面解吸。通过利用这种简单的吸附和解吸机制，细胞可以在寡肽功能化的金表面上生长，并可以通过施加负电位有效地分离为单个细胞或细胞片。这种方法也适用于镀金微棒的表面。通过将内皮细胞转移到微通道的内表面，在胶原凝胶中形成毛细管样微通道。在随后的灌注培养过程中，包膜内皮细胞迁移到胶原凝胶中并形成管腔结构，该管腔结构从微通道中发芽。该技术有潜力为厚细胞片以及血管化组织和器官的工程提供基本工具。版权所有 (c) 2011 John Wiley & Sons, Ltd.
This paper describes a non-invasive approach for efficient detachment of cells adhered to a gold substrate via a specific oligopeptide. Detachment is effected by an electrical stimulus. The oligopeptide contains cysteine, which spontaneously forms a goldthiolate bond on a gold surface. This chemical adsorption reaches>95% equilibrium within 10min after immersion of a gold-coated substrate in a solution containing the peptide. The peptide is reversibly desorbed from the surface within 5min of application of a negative electrical potential. By taking advantage of this simple adsorption and desorption mechanism, cells can be grown on an oligopeptide-functionalized gold surface and can be efficiently detached as single cells or cell sheets by application of a negative electrical potential. This approach was also applied to the surface of gold-coated microrods. Capillary-like microchannels were formed in collagen gel by transferring endothelial cells to the internal surfaces of the microchannels. During subsequent perfusion culture, the enveloped endothelial cells migrated into the collagen gel and formed luminal structures, which sprouted from the microchannels. This technique has the potential to provide a fundamental tool for the engineering of thick cell sheets as well as vascularized tissues and organs. Copyright (c) 2011 John Wiley & Sons, Ltd.