Circulating lncRNA NEAT1 correlates with increased risk, elevated severity and unfavorable prognosis in sepsis patients
Circulating lncRNA NEAT1 correlates with increased risk, elevated severity and unfavorable prognosis in sepsis patients
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DOI：
10.1016/j.ajem.2018.06.008
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发表时间：
2018-09-01
影响因子：
3.6
通讯作者：
Zhang, Rongfang
中科院分区：
文献类型：
--
作者：
Huang, Qinghe;Huang, Cuiyu;Zhang, Rongfang
目的：探讨脓毒症患者循环长非编码RNA核富集丰富转录物1（lncRNA NEAT1）表达与疾病风险、严重程度、预后和炎性细胞因子水平的相关性。方法：纳入152例脓毒症患者和150名健康对照者（HC）。采集脓毒症患者和HCs的血浆和血清样本，采用定量聚合酶链反应法检测血浆中lncRNA NEAT1的表达量，采用酶联免疫吸附法检测血清中炎性细胞因子的水平。结果：脓毒症患者中LncRNA NEAT1的表达量显着高于HCs（P<0.001）。受试者工作特征（ROC）曲线显示lncRNA NEAT1表达对脓毒症风险具有良好的预测价值，曲线下面积（AUC）为0.730（95% CI：0.740-0.861）。随后的多变量逻辑回归分析表明，lncRNA NEAT1 表达与较高的脓毒症风险独立相关（P < 0.001）。在脓毒症患者中，lncRNA NEAT1表达还与急性生理学和慢性健康评估（APACHE）II评分（P < 0.001）、血清肿瘤坏死因子-α（P < 0.001）、白细胞介素（IL）-1 beta（P - 0.043）、IL-6（P = 0.001）和IL-8（P = 0.038），而与 IL-10 呈负相关（P < 0.001）。此外，与幸存者相比，非幸存者中的lncRNA NEAT1表达增加（P - 0.006），ROC曲线显示lncRNA NEAT1对非幸存者风险具有良好的预后价值，AUC为0.641（95% CI：0.536-0.746）。结论：循环lncRNA NEAT1与疾病风险增加、严重程度升高和疾病风险增加相关。 脓毒症患者的预后不良以及促炎细胞因子的表达较高。 (C) 2018 年由爱思唯尔公司出版
Objective: To investigate the correlation of circulating long non-coding RNA nuclear-enriched abundant transcript 1 (lncRNA NEAT1) expression with disease risk, severity, prognosis and inflammatory cytokine levels in sepsis patients.Methods: 152 sepsis patients and 150 health controls (HCs) were enrolled in this study. Plasma and serum samples were obtained from sepsis patients and HCs, and lncRNA NEAT1 expression in plasma was determined by quantitative polymerase chain reaction, while levels of inflammatory cytokines in serum were detected by enzyme linked immune sorbent assay.Results: LncRNA NEAT1 expression was remarkably higher in sepsis patients than in HCs (P< 0.001). Receiver operating characteristic (ROC) curve disdosed a good predictive value of lncRNA NEAT1 expression for sepsis risk with area under curve (AUC) of 0.730 (95% CI: 0.740-0.861). Subsequent multivariate logistic regression analysis demonstrated that lncRNA NEAT1 expression was independently associated with higher sepsis risk (P < 0.001). In sepsis patients, lncRNA NEAT1 expression was also observed to be positively correlated with Acute Physiology and Chronic Health Evaluation (APACHE) II score (P < 0.001), serum tumor necrosis factor-alpha (P < 0.001), interleukin (IL)-1 beta (P - 0.043), IL-6 (P = 0.001) and IL-8 (P = 0.038), while negatively correlated with IL-10 (P < 0.001). In addition, lncRNA NEAT1 expression was increased in non-survivors compared to survivors (P - 0.006), and ROC curve revealed a good prognostic value of lncRNA NEAT1 for non-survivor risk with AUC 0.641 (95% CI: 0.536-0.746).Conclusion: Circulating lncRNA NEAT1 correlates with increased disease risk, elevated severity and unfavorable prognosis as well as higher expression of pro-inflammatory cytokines in sepsis patients. (C) 2018 Published by Elsevier Inc.