Phylogenetic analyses of the isonychiid mayflies (Ephemeroptera: Isonychiidae) in the northeast palearctic region
Phylogenetic analyses of the isonychiid mayflies (Ephemeroptera: Isonychiidae) in the northeast palearctic region
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DOI：
10.1111/1748-5967.12168
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发表时间：
2016-07-01
影响因子：
1.3
通讯作者：
Tojo, Koji
中科院分区：
文献类型：
--
作者：
Saito, Rie;Jo, JaeIck;Tojo, Koji
本文研究了分布于东古北界的Isonychia（Isonychia）japonica、Isonychia（Isonychia）acutta、Isonychia（Isonychia）ussurica和Isonychia（Prinoides）shima的等甲科蜉蝣的分子系统发育。讨论了它们的遗传结构、发生学和地理分布。在东北古北区（日本列岛、朝鲜半岛、俄罗斯远东和蒙古）的47个地点共采集到100种等甲蜉蝣标本。我们分析了mtDNA COI和16S rRNA区域以及nuDNA组蛋白H3区域的DNA序列。通过对东北古北界四种蜉蝣的遗传分析，发现它们在种水平上的单系性得到了mtDNA（COI和16S rRNA区域）和nuDNA（组蛋白H3区域）的支持。此外，还发现在种间水平上存在着显著的遗传分化，因此支持了shima+（japonica+（银杉属+乌苏里））的关系。东北古北界四种等甲蜉蝣中，I.（P。）shima在所包括的种中是一个最基部的谱系
We investigated the molecular phylogeny of isonychiid mayflies inhabiting the East Palearctic region, Isonychia (Isonychia) japonica, Isonychia (Isonychia) ignota, Isonychia (Isonychia) ussurica and Isonychia (Prinoides) shima. We discuss their genetic structures, phylogeny and phylogeography. We collected a total of 100 specimens of isonychiid mayfly species from 47 localities of the Northeast Palearctic region (the Japanese archipelago, the Korean peninsula, the Russian Far East and Mongolia). We analyzed the DNA sequences at the mtDNA COI and 16S rRNA regions, and the nuDNA Histone H3 region. As a result of our genetic analyses of the four Northeast Palearctic isonychiid mayflies, their monophyly at the species level was supported by both the mtDNA (COI and 16S rRNA regions) and the nuDNA (Histone H3 region). In addition, it also became clear that significantly large genetic differentiation exists at the inter-species level; thus, the relationship of shima+(japonica+(ignota+ussurica)) is supported. Among the four isonychiid mayflies of the Northeast Palearctic area, I. (P.) shima was shown to be a basal-most linage within the included species