Molecular genetic analysis of sperm competition in the damselfly Ischnura elegans (Vander Linden)
Molecular genetic analysis of sperm competition in the damselfly Ischnura elegans (Vander Linden)
复制标题
DOI：
10.1098/rspb.1996.0197
复制
发表时间：
1996-10-22
影响因子：
4.7
通讯作者：
Holland, PWH
中科院分区：
文献类型：
--
作者：
Cooper, G;Miller, PL;Holland, PWH
在交配系统的进化中，精子竞争可能是一种强大的选择力量。一些有齿的物种吸引了人们的研究，以评估最后一个雄性精子优先的程度和机制。IschNura(Zygoptera)属的成员表现出一系列特别有趣的交配系统，IschNura elegans被选为研究对象。对微卫星进行克隆、测序，并用于确定线虫幼虫的亲缘关系，以揭示精子优先的模式。从野生和人工饲养的线虫雌体中收集了3000多头幼虫，对其进行了一个或两个微卫星座位的分型，并通过与亲本的比较来确定亲子关系。微卫星分型显示，大多数野生捕获的雌性曾与几只雄性交配。对圈养交配的雌性后代的分析表明，多次交配会导致很大比例的最后一只雄性精子优先(最近最后一只雄性优先的平均值为0.79+/-0.2(+/-S.D.；n=11，范围=0.44-1))。在直接优先和长期优先的程度和模式上有明显的差异，这可能反映了男性精子去除能力或女性对精子的选择性使用的差异。
Sperm competition can be a powerful selective force in the evolution of mating systems. Several odonate species have attracted study to assess the extent and mechanism of last-male sperm precedence. Members of the genus Ischnura (Zygoptera) display a particularly interesting range of mating systems, and Ischnura elegans was selected for study. Polymorphic microsatellites were cloned, sequenced and used to determine paternity of I. elegans larvae, to reveal patterns of sperm precedence. More than 3000 larvae, collected from both wild and captively bred I. elegans females, were typed for one or two microsatellite loci and paternity was determined by comparison with parental genotypes. Microsatellite typing showed that most wild-caught females had mated with several males. Analysis of offspring from females which mated in captivity showed that multiple-matings result in a large proportion of last-male sperm precedence (mean value for immediate last male precedence is 0.79+/-0.2 (+/-s.d.; n = 11, range = 0.44-1)). There is appreciable variation in the extent and patterns of immediate and longer-term precedence, which could reflect differences in male sperm removal ability or selective use of sperm by females.