Resistance exercise decreases skeletal muscle tumor necrosis factor α in frail elderly humans
Resistance exercise decreases skeletal muscle tumor necrosis factor α in frail elderly humans
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DOI：
10.1096/fj.00-0274com
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发表时间：
2001-02-01
期刊：
影响因子：
4.8
通讯作者：
Semenkovich, CF
中科院分区：
文献类型：
--
作者：
Greiwe, JS;Cheng, B;Semenkovich, CF
骨骼肌的蛋白质和功能随着年龄的增长而下降，但其潜在的病理生理机制尚不清楚。为了验证分解代谢细胞因子肿瘤坏死因子α（TNF-α）有助于这一过程的假设，我们研究了衰老和抗阻运动对人类肌肉中TNF-α表达的影响。使用原位杂交，TNF-ar信息被定位于老年人骨骼肌切片中的肌细胞。从虚弱的老年人（81+/-1岁）到健康的年轻人（23+/-1岁）男性和女性的骨骼肌中TNF-α mRNA和蛋白水平都升高。为了确定抗阻运动是否影响TNF-α的表达，将体弱的老年男性和女性随机分配到训练组或非运动对照组。在3个月前和3个月后进行肌肉活检。运动组的肌肉TNF-α mRNA和蛋白水平下降，但对照组没有变化。运动组的肌肉蛋白质合成率与TNF-α蛋白水平呈负相关。这些数据表明，TNF-α有助于与年龄相关的肌肉萎缩和阻力运动可以通过抑制骨骼肌TNF-α的表达减弱这一过程。
Skeletal muscle protein and function decline with advancing age but the underlying pathophysiology is poorly understood. To test the hypothesis that the catabolic cytokine tumor necrosis factor alpha (TNF-alpha) contributes to this process, we studied the effects of aging and resistance exercise on TNF-alpha expression in human muscle. Using in situ hybridization, TNF-ar message was localized to myocytes in sections of skeletal muscle from elderly humans. Both TNF-alpha mRNA and protein levels were elevated in skeletal muscle from frail elderly (81+/-1 year) as com pared to healthy young (23+/-1 year) men and women. To determine whether resistance exercise affects TNF-alpha expression, frail elderly men and women were randomly assigned to a training group or to a nonexercising control group. Muscle biopsies were performed before and after 3 months. Muscle TNF-alpha mRNA and protein levels decreased in the exercise group but did nor change in the control group. Muscle protein synthesis rate in the exercise group was inversely related to levels of TNF-ol protein. These data suggest that TNF-alpha contributes to age-associated muscle wasting and that resistance exercise may attenuate this process by suppressing skeletal muscle TNF-alpha expression.