ULTRASTRUCTURAL EVIDENCE THAT CHICK PRIMORDIAL GERM-CELLS LEAVE THE BLOOD-VASCULAR SYSTEM PRIOR TO MIGRATING TO THE GONADAL ANLAGEN
ULTRASTRUCTURAL EVIDENCE THAT CHICK PRIMORDIAL GERM-CELLS LEAVE THE BLOOD-VASCULAR SYSTEM PRIOR TO MIGRATING TO THE GONADAL ANLAGEN
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DOI：
10.1111/j.1440-169x.1983.00345.x
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发表时间：
1983-01-01
影响因子：
2.5
通讯作者：
FUJIMOTO, T
中科院分区：
文献类型：
--
作者：
ANDO, Y;FUJIMOTO, T
鸡原始生殖细胞(PGC)在胚胎发育早期从内胚层分离后，通过血管系统暂时循环，最终迁移到性腺原。缺乏循环中的PGC离开血管的直接证据。为了描述PGC从血管中冒出时的超微结构特征，首先用甲苯胺蓝染色的半薄切片观察了离开血管的PGC。然后将其中一些切片重新嵌入EPON 812中，并对其进行EM切片。胎儿期15-18(Apprx)，内脏中胚层与开肠内胚层之间的脐肠系膜动脉后区域的毛细血管内可见PGC。孵化2.5天)。在超微结构上，PGC通过毛细血管壁内皮细胞间隙表现出突出的、隆起的细胞质突起。在出现之前，血管内PGC似乎附着在血管内皮细胞上。结果提供了超微结构证据，表明循环中的PGC在迁移到性腺原之前离开血管。
Chick primordial germ cells (PGC), after separation from the endoderm in early embryonic development, temporarily circulate via the blood-vascular system and eventually migrate to the gonadal anlagen. Direct evidence that circulating PGC leave the blood vessels is lacking. To describe the ultrastructural features of PGC as they emerge from the blood vessels, PGC leaving the blood vessels were first examined with semi-thin sections stained with toluidine blue. Then some of the sections were re-embedded in Epon 812 and sectioned for EM. PGC were observed emerging from the capillaries in the region posterior to the omphalomesenteric arteries of the embryo, between the splanchnic mesoderm and open-gut endoderm, at stages 15-18 (.apprx. 2.5 days of incubation). Ultrastructurally, PGC exhibited the protruding, bulge-like cytoplasmic processes through the endothelial gaps in the capillary walls. Prior to emerging, intravascular PGC seemed to stick to the endothelium of the blood vessels. Results offer ultrastructural evidence that the circulating PGC exit the blood vessels prior to migrating to the gonadal anlagen.