In vitro and in vivo growth inhibition of SC-M1 gastric cancer cells by retinoic acid.
In vitro and in vivo growth inhibition of SC-M1 gastric cancer cells by retinoic acid.
复制标题
视黄酸对 SC-M1 胃癌细胞的体外和体内生长抑制作用。
作者：
S. Jiang;R. Shyu;H. Chen;M. M. Lee;K. Wu;M. Yeh
类维生素A是一种分化剂，已成功用于治疗急性早幼粒细胞白血病。当与干扰素联合使用时，它们在预防头颈癌患者的第二次恶性肿瘤方面是有效的。我们前期的研究表明全反式维甲酸（alltrans-retinoic acid，tRA）对体外培养的SC-M1胃癌细胞有抑制作用。本研究比较了tRA和13-cis-维甲酸（cRA）对SC-M1细胞的体内、外作用。使用总细胞DNA的测量来确定体外细胞生长。通过将缓释tRA或cRA颗粒植入无胸腺裸鼠中来评估视黄酸对肿瘤生长的影响。结果表明，tRA在体外对SC-M1胃癌细胞的生长抑制作用强于cRA。tRA和cRA均能有效抑制裸鼠SC-M1肿瘤的生长。在切除的肿瘤中未观察到分化状态和细胞周期时相分布的变化。在两个治疗组的小鼠中均观察到骨折和体重减轻等副作用。结果表明，维甲酸可能为胃癌的治疗提供治疗优势。
Retinoids are differentiating agents that have been used successfully for the treatment of acute promyelocytic leukemia. When combined with interferons, they are active in preventing second malignancies in patients with head and neck cancer. Our previous studies have demonstrated cytostatic effects of alltrans-retinoic acid (tRA) on SC-M1 gastric cancer cells in vitro. The activity of tRA and 13-cis-retinoic acid (cRA) on SC-M1 cells was compared both in vitro and in vivo in this study. Measurement of total cellular DNA was used to determine cell growth in vitro. The effect of retinoic acid on tumor growth was evaluated by implanting sustained release tRA or cRA pellets into athymic nude mice. The results showed that tRA was more potent than cRA in suppressing the growth of SC-M1 gastric cancer cells in vitro. Both tRA and cRA were effective in suppressing the growth of SC-M1 tumors in athymic nude mice. No change in the differentiation status and cell cycle phase distribution in excised tumors was observed. Side effects such as bone fractures and weight loss were observed in mice of both treatment groups. The results suggest that retinoic acid may provide therapeutic advantages for the treatment of gastric cancer.